PřF:C8102 Special methods - laboratory - Course Information
C8102 Special methods - laboratory
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 0/0/5. 5 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
- Teacher(s)
- doc. Mgr. Karel Novotný, Ph.D. (seminar tutor)
Mgr. Aleš Hrdlička, Ph.D. (seminar tutor)
doc. Mgr. Tomáš Vaculovič, Ph.D. (seminar tutor)
RNDr. Marta Farková, CSc. (seminar tutor)
Mgr. Miroslava Bittová, Ph.D. (seminar tutor)
doc. RNDr. Jiří Urban, Ph.D. (seminar tutor)
prof. Mgr. Jan Preisler, Ph.D. (seminar tutor)
Mgr. Lucie Šimoníková (seminar tutor)
Mgr. Antonín Bednařík, Ph.D. (seminar tutor) - Guaranteed by
- doc. Mgr. Karel Novotný, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Karel Novotný, Ph.D.
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science - Prerequisites
- Following lectures is appropriate attend before or during exercise: C7021 Separation Methods A, C7031 Analytical Atomic Spectrometry, C7041 Molecular Spectrometry, C7050 Electroanalytical Methods, C7830 Capillary Electrophoresis and C7895 Mass Spectrometry of Biomolecules.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 9 fields of study the course is directly associated with, display
- Course objectives
- Main objectives can be summarized as practice training in electroanalytical methods, optical spectroscopy, mass spectroscopy, and separation methods. A) Modern electroanalytical methods, potenciometry with ion selective electrode, elimination and stripping voltammetry. B) UV/VIS spectrophotometry, atomic absorption spectrometry (AAS), atomic emission spectrometry with inductively coupled plasma (ICP-OES), laser spectrometry (LA-ICP-OES, LIBS). C) Isotachophoresis, high performance liquid chromatography (HPLC), capillary chromatography on monolithic stationary phases, capillary zone electrophoresis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS).
- Learning outcomes
- Obtaining theoretical knowledge and practical skills in selected analytical methods. Students get to know with hi-tech instrumentation used for research and development of advanced analytical techniques.
- Syllabus
- A) Introduction to course: electroanalytical methods, spectroscopy, separation methods. B) ELECTROANALYTICAL METHODS 1. Nitrate determination by ion-selective electrode in the presence of interfering ion. Nikolski equation. Determination of selectivity coefficient by constant interferent concentration method. 2. Elimination Voltammetry with Linear Scan (EVLS), potential-close signals separation (monitoring of reduction and oxidation on different electrodes). C) SPECTRAL METHODS 3. UV / VIS molecular spectroscopy. Multicomponent spectroscopic and kinetic analysis. 4.Atomic absorption spectrometry with electrothermal atomization 5. ICP OES/MS spectrometry. Methods for decomposition of samples: cryogenic grinding, microwave decomposition. Solution analysis: ICP OES and ICP MS spectrometry. 6. Analytical methods based on laser ablation (LA ICP MS). Laser-induced breakdown spectroscopy (LIBS): surface mapping. D) SEPARATION METHODS 7. Determination of anions by ITP 8. Liquid chromatography (HPLC) - Determination of inosine, adenosine and 2'-deoxy-forms in the model mixture - optimization and validation of the method. 9. Polyacrylamide gel electrophoresis (SDS-PAGE). Separation of proteins in a discontinuous GE system. Subsequent visualization by CBB R 250 and silver. Identification of model proteins in a mixture. 10. CE-LIF, capillary zone electrophoresis with laser-induced fluorescence detection. Optimization of experimental setup. Determination of the detection limit for Rhodamine 6G. Separation of rhodamine dyes. 11. Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS). Selected applications of MALDI MS: calibration, determination of molecular weight, peptide mapping, identification of unknown protein. Links to information for individual tasks: http://www.sci.muni.cz/~labifel/?q=studium_materialy http://www.chemi.muni.cz/~lpca/skripta2.html http://www.is.muni.cz/el/1431/jaro2010/C8102 http://bart.chemi.muni.cz/courses.htm
- Literature
- recommended literature
- CHURÁČEK, Jaroslav. Nové trendy v teorii a instrumentaci vybraných analytických metod. Vyd. 1. Praha: Academia, 1993, 387 s. ISBN 8020000100. info
- KANICKÝ, Viktor, Vítězslav OTRUBA, Lumír SOMMER and Jiří TOMAN. Optická emisní spektrometrie v indukčně vázaném plazmatu a vysokoteplotních plamenech (Optical emission spectrometry in inductiveky coupled plasma and high temperature flames). 1. st. Praha: Academia, 1992, 152 pp. Pokroky chemie 24. ISBN 80-200-0215-4. info
- CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990, 384 s. ISBN 80-03-00569-8. info
- SOMMER, Lumír. Analytical absorption spectrophotometry in the visible and ultraviolet : the principles. Amsterdam: Elsevier, 1989, 310 s. ISBN 0-444-98882-38. info
- Analytická příručka. Edited by Jaroslav Zýka. 4., upr. vyd. Praha: SNTL - Nakladatelství technické literatury, 1988, 831 s. info
- Analytická příručka. Díl I [Zýka, 1988]. Edited by Jaroslav Zýka. 4. upr. vyd. Praha: SNTL - Nakladatelství technické literatury, 1988, 678 s. info
- Pokroky v teorii a instrumentaci moderních analytických metod. Edited by Jaroslav Churáček. 2. přeprac. a dopl. vyd. Pardubice: Vysoká škola chemicko-technologická, 1988, 193 s. info
- HOLZBECHER, Záviš and Jaroslav CHURÁČEK. Analytická chemie. 1. vyd. Praha: Státní nakladatelství technické literatury, 1987, 663 s. info
- SOMMER, Lumír. Analytická spektrometrie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1986, 173 s. info
- KORYTA, Jiří. Současné trendy v elektrochemii. 1. vyd. Praha: Academia, 1986, 128 s. info
- Nové směry v analytické chemii. Edited by Jaroslav Zýka. 1. vyd. Praha: Státní nakladatelství technické literatury, 1984, 218 s. info
- BARTUŠEK, Miloš. Úvod do elektroanalytických metod. 1. vyd. Praha: SPN, 1984, 104 s. : i. info
- CHURÁČEK, Jaroslav. Úvod do vysokoúčinné kapalinové kolonové chromatograrie. Edited by Pavel Jandera. Vyd. 1. Praha: SNTL - Nakladatelství technické literatury, 1984, 188 s. info
- DVOŘÁK, Jiří and Jiří KORYTA. Elektrochemie. 3., dopl. a rozš. vyd. Praha: Academia, 1983, 410 s. URL info
- Nové směry v analytické chemii. Edited by Jaroslav Zýka. 1. vyd. Praha: Státní nakladatelství technické literatury, 1983, 199 s. info
- CHURÁČEK, Jaroslav and Pavel JANDERA. Separace látek : kapalinová vysokoúčinná kolonová chromatografie. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1981, 140 s. info
- SOMMER, Lumír. Teorie a praxe vybraných optických analytických metod. 1. vyd. Praha: Státní pedagogické nakladatelství, 1978, 285 s. info
- Teaching methods
- laboratory courses
- Assessment methods
- Three closed cycles of laboratory course: electroanalytical methods, optical methods, separation methods (+MALDI - MSTOF). Preliminary announcement to lector is necessary. Graded credit requirements: completion of all laboratory exercises
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
General note: dle oboru.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/C8102