PřF:C9100 Biosensors - Course Information
C9100 BiosensorsFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Taught in person.
- prof. RNDr. Petr Skládal, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
- Mon 11:00–12:50 B11/335
- basic overview of biochemistry
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 23 fields of study the course is directly associated with, display
- Course objectives
- Students will gain overview of biosensors, including electrochemical and optical sensors. The experience on sensors based on affinity interactions as surface plasmon resonance (SPR) and piezoelectric transducers will serve for characterization of affinity interactions. The obtained knowledge will help to understand principles of the instrumentation available at the Faculty of Science and CEITEC MU. Students will learn applications of biosensors in clinical analysis, environmental monitoring, food production and security systems.
- Learning outcomes
- Student will learn principles of biocatalytic and affinity biosensors, properties of enzymes, antibodies and cells as biorecognition elements. Student will gain basic knowledge on the immobilization of biomolecules and applications of biosensors in clinical medicine, food industry, military and biotechnologies.
- 1. Definition of the biosensor. Historic overview. Characteristics of ideal biosensors. Basic measuring techniques. 2. Electrochemical biosensors, enzyme electrodes. Potentiometric systems and ISFETs. Reference electrodes. 3. Amperometric measurement of oxygen, hydrogen peroxide and NADH, biosensors based on oxidases and dehydrogenases. 4. Electron transfer from enzymes to electrodes using mediators. Composite and organometallic electrodes. 5. Impedimetric and conductometric biosensors. Voltammetric techniques 6. Spectrophotometric, fluorimetric a chemiluminescent sensors, optical fibers. Optical biocatalytic sensors. Bioluminescence. 7. Biosensors for detection of inhibitors. Recycling enzyme systems. Microbial, tissue and receptor-based sensors. 8. Affinity biosensors based on indirect detection of labels. Immunosensors. 9. Hybridization biosensors for detection of nucleic acids and oligonucleotide sequences. 10. Direct optical affinity sensors. Evanescent wave and surface plasmon resonance utilized for monitoring of bioaffinity interactions in real time. Integrated optical systems, interferometers and related techniques. 11. Immobilization of biomolecules for construction of biosensors. Membrane techniques. Electropolymerization. 12. Activation of sensing surfaces. Covalent immobilization of biomolecules. 13. Miniaturization and mass production of biosensors. Screen-printing, litography and mass production of biosensors. Biosensors in integrated analytical microsystems. Biochips. 14. Commercial biosensors. Perspectives of biosensors, applications in clinical medicine, food production, environmental monitoring, military application.
- Teaching methods
- lecture course
- Assessment methods
- Participation at lectures required, oral examination - random selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
- Language of instruction
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
- Teacher's information
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2022/C9100