PřF:B5491 Molecular biology - practice 1 - Course Information
B5491 Molecular Biology - Practice 1
Faculty of ScienceAutumn 2000
- Extent and Intensity
- 0/8/0. 9 credit(s). Type of Completion: graded credit.
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. RNDr. Jan Šmarda, CSc. (seminar tutor) - Guaranteed by
- doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Course objectives
- Preparation of solutions used in molecular biology. Isolation spontaneous mutants of Staphylococcus aureus resistant to streptomycine. One step growth curve of bacteriophage. Fluctuation test. Replica-plating of colonies a plaques. Isolation of plasmid DNA; Determination purity and concentration of DNA; Digestion of DNA by restriction endonucleases; Horizontal slab gel electrophoresis of DNA in agarose gels; Cultivation of eucaryotic cell cultures. Cytocentrifugation of influenced eucaryotic cells; Isolation of proteins; SDS-page methods.
- Language of instruction
- Czech
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
- Enrolment Statistics (Autumn 2000, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2000/B5491