C5910 Chromatographic Methods I.

Faculty of Science
Autumn 2008
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Tue 9:00–10:50 kamenice
Prerequisites
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 15 fields of study the course is directly associated with, display
Course objectives
Introductory part deals with chromatographic terms, chromatogram evaluation, and theory of chromatographic separation. Description of principles, techniques, instrumentation, and contemporary trends in the field of high performance liquid chromatography (HPLC) and high performance thin-layer chromatography (HPTLC) follows. The end of course is foccused on trends in development of chromatographic techniques and examples and comparison of chromatographic methods on selected groups of compounds and types of analyzis. Capillary electrophoresis (HPCE) and electrochromatography (CEC) as well as gas chromatography (HRGC), are discussed in Chromatographic Methods II course. Main objectives are: to understand the chromatograoic principles; to obtain detailed the information on teory of chromatographic separation; to learn the development and actual state of chromatographic instrumentation; to obtain information necessary for laboratory use. chromatografických metod.
Syllabus
  • 1. Chromatographic separation, retention parameters, chromatogram, measure of separatio, separation efficiency. chromatographic zones.van Deemter equation a Golay equation. Retention indexes.
  • 2. Gas chromatography.
  • 3. Gas-liquid chromatography. Columns, liquid phases, carriers.
  • 4. Adsorption gas chromatography. Characteristics , comparison with GLC. Adsorbents, aplikation.
  • 5. Kapiláry column, WCOT, PLOT, SCOT. Quality evaluation.
  • 6. Mobile phases, gas mobile phases comparison.
  • 7. GC instrumentation, sample injection, detection.
  • 8. Liquid chromatography. Stacionáry and mobile phases, separation properties
  • 9. Column, stationary phases, sorbents properties.
  • 10. Mobile phases, clasification of solvents, elution techniques.
  • 11. Techniques, principples, retention models, separation strategy, aplikation.
  • 12. Instrumentation, pumps, injection devices, detectors and detection principles.
  • 13. Trendy in development of chromatographic methods.
  • 14. Examples of chromatographic aplications.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • MEYER, Veronika R. Practical High-Performance Liquid Chromatography. 3rd ed. Chichester: J. Wiley & Sons, 1999, 338 pp. ISBN 0-471-98372-1. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
Assessment methods
lectures, oral exam
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2008, recent)
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