Bi7900 Genetic methods in zoology

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Miloš Macholán, CSc. (lecturer)
prof. Mgr. et Mgr. Josef Bryja, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Miloš Macholán, CSc.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Miloš Macholán, CSc.
Prerequisites (in Czech)
Bi1030 Syst. & evol. of invertebrates && Bi2090 System evolution vertebrates && Bi3060 Basic genetics && Bi4020 Molecular biology || SOUHLAS
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to give undergraduate and PhD students a basic orientation in principles of morphological, cytogenetic, and molecular methods currently used in zoological research. After finishing the course, students should gain theoretical knowledge and practical skills in the following topics: Analysis of phenotype (epigenetic traits, quantitative traits, geometric morphometrics); Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting“); Protein and DNA electrophoresis; DNA analysis (DNA isolation, DNA-DNA hybridization, PCR, real-time PCR, fingerprinting, RAPD, AFLP, analysis of microsatellites, LINE, SINE, and SNP; sequencing, "next generation sequencing, RFLP, DGGE, TGEE, SSCP, cloning, microarrays); Methods of analysis (population-genetic data: analyses based on allele frequencies vs. "individual-based" models; phylogenetic data: distance methods, maximal parsimony, maximal likelihood, Bayesian analysis).
Syllabus
  • 1. Analysis of phenotype (signal phenotypes, epigenetic traits, quantitative traits, landmark analysis)
  • 2. Cytogenetics (karyotype analysis, banding, FISH, „ chromosome painting“) and elektroforetic methods (proteins, DNA)
  • 3. DNA aAnalysis I (DNA isolation, genetic markers - nuclear vs. cytoplasmic DNA, DNA-DNA hybridization, PCR, real-time PCR, "multi-locus" DNA markers: fingerprinting, RAPD, AFLP)
  • 4. DNA analysis II ("single-locus" DNA markery: mikrosatelity, LINE, SINE, SNP)
  • 5. DNA analysis III (sequencing methods, "next generation sequencing") 6. DNA analysis IV (SNP and their analysis: RFLP, DGGE, TGEE, SSCP, cloning, microarrays)
  • 7. Methods of analysis I (population-genetic data) - analyses based on allele frequencies vs. "individual-based" models
  • 8. Methods of analysis II (phylogenetic data) - distance methods, maximal parsimony, maximal likelihood, Bayesian methods
  • 9. Practical class I (chromosomal preparations, karyotype analysis)
  • 10. Practical class II (protein electrophoresis)
  • 11. Practical class III (PCR, real-time PCR)
  • 12. Practical class IV (analysis of DNA fragments after PCR, electrophoresis, fragment analysis, capillary SSCP)
  • 13. Practical class V(sequencing)
Literature
  • ZIMA, Jan. Genetické metody v zoologii. Online. 1. vyd. Praha: Karolinum, 2004. 239 s. ISBN 8024607956. [citováno 2024-04-23] info
Assessment methods
lectures, practical classes;
oral exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2003, Spring 2004, Spring 2005, Spring 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.