PřF:Bi6882 Biomarkers - Course Information
Bi6882 Biomarkers and toxicity mechanismsFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- prof. RNDr. Luděk Bláha, Ph.D. (lecturer)
- Guaranteed by
- prof. RNDr. Luděk Bláha, Ph.D.
RECETOX - Faculty of Science
Contact Person: prof. RNDr. Luděk Bláha, Ph.D.
Supplier department: RECETOX - Faculty of Science
- Mon 17. 9. to Fri 14. 12. Wed 14:00–15:50 D29/252-RCX1
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Course objectives
- At the end of the course students will be able to:
- understand major mechanisms of effects of foreign compounds in the organisms and the applications as the biomarkers.
- define the most important biochemical and cellular processes involved in genotoxic as well as non-genotoxic effects.
- recognize and classify major classes of xenobiotics with the focus on major classes of environmental contaminants, newly emerging cytostatic and chemoprotective drugs.
- discuss and interpret biochemical markers of susceptibility, exposure and effects.
- use experimental methods of in vivo and in vitro biomarkers.
- know examples of practical applications of biomarkers in toxicology, ecotoxicology, and molecular epidemiology
- 1. Mechanisms of toxicity and biomarkers - introduction to effects of xenobiotics in organisms, toxokinetics and toxodynamics. Effects at different organismal levels - importance of biochemical processes. Biomarkers of exposure, effects and susceptibility. 2. Major classification of adverse effects (genotoxicity vs. non-genotoxic mechanisms, organ specific toxicity). Approaches and principles of the studies in vivo and in vitro. 3. Metabolism of xenobiotics. Drug-metabolising enzymes, activation and detoxification. Cytochromes P450 - classes, polymorphism, induction and toxicological importance. Enzymes of phase II of biotransformations. Antioxidant compounds and processes. Biomarkers of exposure and susceptibility. 4. Genotoxic effects. DNA and its importance, mutations and other adverse chemically induced genotoxic effects. Strategies, possibilities and methods of genotoxicity testing. Genotoxicity of xenobiotics in risk assessment. Biomarkers of exposure and effects. 5. General cytotoxicity - necrosis. Common mechanisms of toxicity in cells, adverse effects in energy metabolism, toxicity to biomembranes, specific mechanisms of cytotoxicity. Methods and studies of cytotoxicity in vitro. 7. Programmed cell death - apoptosis. Importance of apoptosis, controlling, possibilities of model modulations of basic processes. Inhibition of apoptosis - an important mechanism of toxicity of xenobiotics. 8. Cell proliferation, modulations of signal transductions, cell cycle. Major processes of non-genotoxic carcinogenesis, transformation and clonal expansion, inhibition of intercellular communication (GJIC). Chemically induced carcinogenesis. 9. Methods for detection and studies of non-genotoxic mechanisms. Dioxin like toxicity, estrogenicity, other mechanisms of endocrine disruption, activation of signalling pathways, oxidative stress, modulation of cell proliferation. Use as biomarkers of exposure and effects. 10. Neurotoxicity and immunotoxicity. Examples and importance of toxic effects, relations of organ-specific toxicity to common biochemical mechanisms of toxic effects. Case studies and methods, specific biomarkers in vivo. 11. Toxicity mechanisms of major xenobiotics: PAHs, PCBs, PCDD/Fs, organochlorine contaminants, detergents, inorganic pollutants, cytostatic and chemoprotective drugs.
- MacGregor J.T. et al.: Strategies and testing methods for identifying mutagenic risks. Mutation Research 455, 3-20, 2000.
- PREEDY, V.R. and V.B. PATEL. General Methods in Biomarker Research and their Applications (Biomarkers in Disease: Methods, Discoveries and Applications). Springer, 2015. info
- KEWAL, K.J. The Handbook of Biomarkers. Humana Press, 2015. info
- Nuclear receptors. Edited by Margaret K. Bates - Regina M. Kerr. Hauppauge, N.Y.: Nova Science, 2011. xiii, 185. ISBN 9781620819715. info
- JANOŠEK, Jaroslav, Klára HILSCHEROVÁ, Luděk BLÁHA and Ivan HOLOUBEK. Environmental xenobiotics and nuclear receptors-Interactions, effects and in vitro assessment. Toxicology in vitro. 2005, vol. 20, No 1, p. 18-37. ISSN 0887-2333. info
- Teaching methods
- Lectures (weekly), supported by e-learning materials in the IS.MUNI.CZ system
- Assessment methods
- The evaluation is based on (i) final written test - 10 open questions (50% success required as minimum), followed by (ii) oral examination (2 broader questions, which test the ability of students to independently think and express themselves). The weights of the written test and oral examination are 70:30%.
- Language of instruction
- Further Comments
- Study Materials
The course is taught annually.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2018/Bi6882