C5911 Chromatographic Methods

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jana Klánová, Ph.D.
RECETOX - Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Supplier department: RECETOX - Faculty of Science
Introductory course on Analytical Chemistry or an equivalent
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to:
- define fundamentals of chromatographic methods and describe them in the context with other separation analytical methods;
- understand and clarify principles of individual types of chromatography and functional layout of chromatographic instrumentation, their individual parts and detecting elements;
- explain theoretical aspects and mechanisms of chromatographic separation and principles of choice of chromatographic systems;
- asses and interpret results of chromatographic analyses;
- evaluate significance of connection of chromatographic methods with various analytical techniques, especially with spectral methods;
- take advantage of chromatography in quantitative and qualitative analyses of various types of samples;
  • 1. Chromatographic separation, retention parameters, chromatogram, measure of separation, separation efficiency. chromatographic zones.van Deemter equation a Golay equation. Retention indexes.
  • 2. Gas chromatography.
  • 3. Gas-liquid chromatography. Columns, liquid phases, carriers.
  • 4. Adsorption gas chromatography. Characteristics , comparison with GLC. Adsorbents, application.
  • 5. Capilary column, WCOT, PLOT, SCOT. Quality evaluation.
  • 6. Mobile phases, gas mobile phases comparison.
  • 7. GC instrumentation, sample injection, detection.
  • 8. Liquid chromatography. Stationary and mobile phases, separation properties
  • 9. Column, stationary phases, sorbents properties.
  • 10. Mobile phases, classification of solvents, elution techniques.
  • 11. Techniques, principles, retention models, separation strategy, application.
  • 12. Instrumentation, pumps, injection devices, detectors and detection principles.
  • 13. Trends in development of chromatographic methods.
  • 14. Examples of chromatographic applications.
  • POOLE, C. F. The essence of chromatography. 1st ed. Amsterdam: Elsevier, 2003. ix, 925. ISBN 0444501991. info
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
  • MEYER, Veronika R. Practical High-Performance Liquid Chromatography. 3rd ed. Chichester: J. Wiley & Sons, 1999. 338 pp. ISBN 0-471-98372-1. info
  • LINDSAY, S. High Performance Liquid Chromatography. 2nd Edit. Chichester: J. Wiley, 1992. Analytical Chemistry by Open Learning (Series). ISBN 0 471 93115 2. info
  • Chromatography 6th edition : fundamentals and applications of chromatography and related differential migration methods. Edited by Erich Heftmann. 1st ed. Amsterdam: Elsevier, 2004. xl, 518. ISBN 0444511067. info
Teaching methods
Education is performed as lectures with Powerpoint presentation. Understanding of mechanisms and consequences is emphasized. Students are frequently asked questions to think about actual topic. They are encouraged to ask questions and be in interaction with the lecturer.
Assessment methods
Attendance of the lectures is not mandatory but strongly recommended for fluent understanding of the educated subjects. Final assessment (at the end of semester) is by oral examination. Tree related topics are the content of the discussion during oral examination.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught each semester.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Spring 2019, Autumn 2019, Autumn 2020.
  • Enrolment Statistics (Autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2018/C5911