PřF:Bi6700c Taxonomy of prok.-lab.course - Course Information
Bi6700c Taxonomy of prok.-lab.course
Faculty of ScienceAutumn 2022
- Extent and Intensity
- 0/4/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Dana Nováková, Ph.D. (seminar tutor)
prof. RNDr. Ivo Sedláček, CSc. (seminar tutor)
doc. RNDr. Pavel Švec, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Ivo Sedláček, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Ivo Sedláček, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- NOW ( Bi6700 Taxonomy of prokaryotes )
Examination - basic microbiology, physiology of bacteria, biochemistry, molecular biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- The main goal of this course is introduction of students in isolation and cultivation of microorganisms and maintenance of pure cultures. The next step is the microscopic and macroscopic morphology of archaea and bacteria, their phenotypic classification and identification and typing of bacteria by rep-PCR method as well as by mass spectrometry MALDI TOF procedure.
- Learning outcomes
- At the end of this course students should be able to perform: - isolation and cultivation of microorganisms, maintenance of pure cultures; - microscopic and macroscopic morphology of archaea and bacteria; - phenotypic classification and identification of Gram negative bacteria; - phenotypic classification and identification of Gram positive bacteria; - typing and identification of bacteria by rep-PCR method; - analysis of microorganisms by mass spectrometry MALDI TOF procedure.
- Syllabus
- Agar and broth media
- Isolation
- Cultivation
- Morphology
- Classification of fermenting rods
- Classification of non-fermenting rods
- Classification of G+ k cocci and rods (phylum Actinobacteria, Firmicutes)
- rep_PCR
- MALDI TOF MS
- Evaluation of identification
- Literature
- required literature
- SEDLÁČEK, Ivo. Taxonomie prokaryot (Taxonomy of prokaryotes). 1st ed. Brno: Masarykova univerzita, 2007, 270 pp. 55-969C-2006 02/58 12Př. ISBN 80-210-4207-9. info
- recommended literature
- Murray, PB., Baron, E.J., Jorgensen, J.H., Pfaller, M.A., Yolken, R.H. (eds.). 2003. Manual of clinical microbioloogy. Volume 1. 8th edition. ASM Press. Washington, D.C.
- Pershing, D.H., Tenover, F.C., Versalovic, J., Tang, Y.W., Unger, E.R., Relman, D.A., White, TJ. (eds.). 2004. Molecular microbiology: diagnostic principles and practice. ASM Press, Washington, USA.
- Teaching methods
- Individual study followed by laboratory practice.
- Assessment methods
- Two written tests before course (30 and 50 questions), lacoratory courses, credit (course).
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
Information on course enrolment limitations: Limitujici je kapacita cvicebny
- Enrolment Statistics (Autumn 2022, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2022/Bi6700c