PřF:C9320 Methods biochem. res. - Course Information

C9320 Methods of biochemical research

Extent and Intensity

0/0/6. 6 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Taught in person.

Teacher(s)

doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. Mgr. Karel Kubíček, PhD. (seminar tutor)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. et Mgr. Lenka Kohútová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)
Mgr. Josef Houser, Ph.D. (seminar tutor)
Mgr. Monika Kubíčková, Ph.D. (seminar tutor)

Guaranteed by

doc. Mgr. Zdeněk Farka, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable of Seminar Groups

C9320/01: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/02: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/03: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/04: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/05: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 12 fields of study the course is directly associated with, display

Course objectives

In the laboratory exercise, students will be acquainted with practical features and basic applications of the methods listed in syllabus.

Learning outcomes

Student will be able:
- to understand basic principles of representative instrumental methods used in biochemistry;
- to use various methods for experimental work in biochemistry and related areas.

Syllabus

• 1. SDS-PAGE
• 2. HPLC
• 3. Biosensors
• 4. Spectrophotometry
• 5. Transfer of oxygen
• 6. RT-qPCR
• 7. Crystallography
• 8. MALDI-MS
• 9. Ultracentrifugation
• 10. NMR
• 11. CZE
• 12. Immunoassays

Literature

recommended literature
• ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR. 199 s. 1986. info

Teaching methods

Individually supervised laboratory course.

Assessment methods

Twelve exercises have to be completed according to the schedule.
Credit completion on the base of participation and protocol processing.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on course enrolment limitations: předmět není možné zapsat v bakalářském programu; v pětiletém magisterském programu je možné předmět zapsat nejdříve ve 4. ročníku

• Enrolment Statistics (Autumn 2022, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2022/C9320