C6202 Omics Technologies (proteomics, genomics, metabolomics)

Faculty of Science
Autumn 2024

The course is not taught in Autumn 2024

Extent and Intensity
0/0/0. Type of Completion: zk (examination).
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
The courses Biochemistry II (C4182en), Molecular biology (Bi4025en) and Introduction to bioinformatics (C2131en)
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The course aims to introduce the use of various omics technologies that aim to collectively characterize pools of biological molecules in a single experiment. The focus is on omics technologies in research today.
Learning outcomes
The student will gain insight into the current technologies available to study the different molecular biological levels (genome/transcriptome/proteome/metabolome). The student will be able to assess their applications and critically evaluate them for specific applications. This will be achieved through specific examples. The student will know and understand current identification approaches (sequence analysis/microarray, mass spectrometry) and will be able to analyze and interpret the biological information obtained based on bioinformatic and experimental approaches.
Syllabus
  • 1st Block - Genome analysis - Overview of genome complexity and mapping approaches - High throughput sequencing analysis techniques, ChIPseq - Genome-scale chemical synthesis approaches for nucleic acids - Bioinformatic analysis of genomes - 16S sequencing and metagenomic analysis 2nd Block - Transcriptome analysis - Techniques in transcriptome analysis - Bioinformatical analysis of RNAseq data - Related biostatistics 3rd Block - Proteome and Metabolome analysis - Protein expression and analysis - Proteome analysis approaches - Principles of mass spectrometry - Peptidomics - Metabolomics - Bioinformatical analysis of proteome data 4th Block - Examples and applications - Pharmacogenomics and genetics - GWAS experiments
Teaching methods
Lecture - Power Point presentations
Assessment methods
Written examination.
Language of instruction
English
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