PřF:Bi2050c General microbiology pr. - Course Information
Bi2050c General microbiology practice
Faculty of ScienceSpring 2009
- Extent and Intensity
- 0/4/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Mgr. Andrea Vávrová, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Danuška Horáková, CSc. - Timetable of Seminar Groups
- Bi2050c/01: Fri 8:00–10:50 Bpb,02012, M. Krsek, A. Vávrová
Bi2050c/02: Fri 11:00–13:50 Bpb,02012, M. Krsek, A. Vávrová - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24 - fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Course objectives
- The main objective of the practical course of general microbiology is to introduce students into basic methods used in microbiological laboratory (methods of sterile work, inoculation and cultivation, microscopic slides preparation and examination of microorganisms, determination of the number of bacteria in different samples, methods of work with bacteriophage, yeasts and microscopic fungi).
- Syllabus
- 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of viable bacteria by plate count 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Determination of the number of phage particles in lysate of bacterial cells 10. Yeasts, test of vitality 11. Microorganisms in soil. Inoculation of microscopic fungi. 12. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 13. Microscopic fungi. 14. Reports, test and microscopic examination/determination of unknown bacterial strain.
- Literature
- JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
- Assessment methods
- practical classes, reports, written test, credit
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2009, recent)
- Permalink: https://is.muni.cz/course/sci/spring2009/Bi2050c