C6850 Chromatographic Methods II.

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Zdeněk Šimek, CSc. (lecturer)
Guaranteed by
prof. RNDr. Ivan Holoubek, CSc.
RECETOX – Faculty of Science
Contact Person: doc. RNDr. Zdeněk Šimek, CSc.
Timetable
Wed 9:00–10:50 kamenice
Prerequisites
Knowledge of principles of chromatograohy corresponding C5910 course, Chromatographic Methods I.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
At the end of the course, students should be able to;
- utilize theory of analytical separation for characterisation and understanding of methods based on chromatographic principles;
- understand and clarify principles of instrumentation and functional layout of thin layer chromatography, methods based on principles of chromatography and analytical electromigration, inversed chromatography and hiphenated separation and related analytical technics;
- asses the possibility of combination of chromatographic and other separation and analytical technics for increasing of identification efficiency and improvement of quantification limits of developed analytical methods;
- aplly theory of chromatography to characterisation of analytical phase systems, surfaces of solid materials and bonded phases and to interaction of analytes with components of phase systems;
Syllabus
  • According to number of semester weeks: 1.- 2. Thin layer chromatography. Principles. Instrumentation. Application.
  • 3.- 6. Capillary electrophoresis and electrochromatography. Ion progression in electrical field, fundamental equations, terms, and parameters. Principles of CE techniques and those of capilllary electrochromatography, applications. Instrumentation. Quantitation.
  • 7.- 8. Inverse chromatography
  • 9.- 10. Hyphenated techniques in separation analysis.
  • 11. - 12. Trends in chromatography. Multidimensional chromatography. Rapid resulution high throuput chromatography. Microseparations.
Literature
  • POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
Assessment methods
lectures, oral exam
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/C6850