FA600 Vibrational spectroscopy of biopolymers

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Dušan Hemzal, Ph.D. (lecturer)
Guaranteed by
Mgr. Dušan Hemzal, Ph.D.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: Mgr. Dušan Hemzal, Ph.D.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Prerequisites (in Czech)
Znalosti základů biofyzikálního kurikula: proteiny, nukleové kyseliny
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
The course intends to introduce usage of vibrational spectroscopy in research of biologicaly important molecules - especially proteins and nucleic acids, including their interactions. The course will emphasize complementarity of vibrational data to other measurement modalities.
Learning outcomes
After passing the course students should be able to
- analyze vibrational spectra of biopolymers
- prepare liquid sample for Raman spectroscopy and obtain its vibrational spectrum
Syllabus
  • Vibrational spectroscopy and its instrumentations: infrared (IR) and Raman (RS) spectroscopies.
  • Molecular vibrations and their analyzis.
  • Vibrational spectra of biomolecules: (oligo)peptides, proteins, nucleic acids, lipids, (poly)saccharides.
  • Modern Raman modalities: resonant Raman scattering (RRS), surface enhanced Raman scattering (SERS), tip enhanced Raman scattering (TERS), vibrational circular diochroism (VCD), Raman optical activity (ROA), droplet coating deposition Raman spectroscopy (DCDR)
  • Other techniques: attenuated total reflection in the infrared (ATR), refractometry, plasmon-enhanced ellipsometry (TIRE) and kinetic studies, scanning probe microscopy (AFM, STM)
Teaching methods
lectures, lab practice
Assessment methods
Oral discussion of the results of the laboratory project devoted to application of vibrational spectroscopy selected by student.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2021/FA600