PřF:Bi6126 aDNA analysis - Course Information
Bi6126 Laboratory sampling for aDNA analysisFaculty of Science
- Extent and Intensity
- 0/2/0. 3 credit(s). Type of Completion: k (colloquium).
- Mgr. Eva Chocholová (seminar tutor)
Mgr. et Mgr. Lenka Falk, Ph.D. (seminar tutor)
Mgr. et Mgr. Kristýna Brzobohatá, Ph.D. (seminar tutor)
doc. RNDr. Eva Drozdová, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Eva Drozdová, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Eva Chocholová
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Bi6400 Methods of molecular biology && Bi6400c Molecular Methods - practice
The course is designed for students of master's degree of Antropobiologie and antropogenetika. Student will learn during the laboratory exercise all steps of aDNA analysis.
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10
- fields of study / plans the course is directly associated with
- Course objectives
- The student gains practical experience with sterile sampling of DNA from historical skeletal material. He will be trained in the work of a DNA sample in a laboratory. This exercise is necessary for students of anthropobiology and anthropogenetics to develop their diploma theses.
- Learning outcomes
- Students completing the course will be able to: - separate tissue sampling for DNA analysis - lysing bone tissue, purifying and DNA - choose the most suitable method for the given topic of the diploma thesis - design a protocol for genetic analysis - optimize a protocol for genetic analysis - bioinformatically and statistically evaluate the results
- 1. Selection of sample for analysis of DNA, anti-contaminating measures 2. Lysis, purification of a DNA 3. Quantification and determination of aDNA quality 4. PCR and its optimization 5. Sequencing 6. Bioinformatics and statistical tools for evaluation of genetic analysis results
- terénní cvičení bez doporučené literatury
- Ancient DNA : recovery and analysis of genetic material from paleontological, archaeological, museum, medical, and forensic specimens. Edited by Bernd Herrmann - Susanne Hummel. New York: Springer-Verlag, 1994. xi, 263. ISBN 0387943080. info
- Teaching methods
- laboratory practise
- Assessment methods
- active participation
- Language of instruction
- Further Comments
- The course is taught annually.
The course is taught: in blocks.