C8004 Advanced Plant Cell Biology

Faculty of Science
Spring 2027
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Mgr. Markéta Pernisová, Ph.D. (lecturer)
Guaranteed by
Mgr. Markéta Pernisová, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: Mgr. Markéta Pernisová, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Prerequisites
Bi4020 Molecular biology
Completion of basic courses of molecular biology and plant cell biology is a prerequisite for subscription to the course. Completion of courses of plant physiology and plant anatomy will be an advantage.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Abstract
The goal of the course is to understand and explain the nature of complex mechanisms and signalling pathways taking place within plant cells, and to correctly interpret their significance for physiological processes in plant tissues, organs and whole organism. Students will understand the advanced structures and physiological processes of plant cells.
Learning outcomes
At the end of the course, students will have an overview of: intracellular and intercellular signalling in plant cells, phytohormonal regulations, intracellular and intercellular transport, special mechanical properties of plant cells, plant cell cycle, plant cell differentiation in intact organism and in vitro, omics approaches on cellular level.
Key topics
  • 1) Signalling pathways of phytohormones: auxin, cytokinins, ethylene, gibberellins, abscisic acid, brassinosteroids, jasmonates, salicylic acid and strigolactones; sensors
  • 2) Intracellular and intercellular transport: cell localization of metabolic enzymes, auxin transport, PIN proteins, PIN polarity and phosphorylation, cytokinin transporters, sugar transporters, plasmodesmata, symplast and apoplast, intercellular transport of nucleic acids
  • 3) Plant cell cycle: cell division as a response to environmental stimuli, plant cell cycle checkpoints, plant nuclear proteins, plastid division, mitochondrial and peroxisomal division, mechanism of organelle inheritance in dividing plant cells, cell cycle in nodulation, cell division affected by pathogens
  • 4) Plant cell enlargement: organization of the plant cell wall, biosynthesis and assembly of cellulose, signalling-dependent cytoskeletal dynamics and plant cell growth, the regulation of plant cell expansion-auxin-induced turgor-driven cell elongation, phytohormonal regulations of cell growth
  • 5) Mechanical properties of plant cells: mechanosensors and signalling, gravity signal perception, amyloplasts, gravity signal transduction, gravity signal transmission, gravitropic growth response
  • 6) Plant stem cells: stem cell of shoot apical meristem, WUS-CLV3 signalling, Ca2+ function, stem cells of root apical meristem, WOX-CLE signalling, phytohormonal regulations, epigenetic regulations, de novo establishment of stem cells – lateral roots, phyllotaxis
  • 7) Plant cell differentiation: asymmetric cell division, polarization, endoreduplication, zygote, embryo, plant vascular tissue development, epidermis cell differentiation
  • 8) In vitro plant cell differentiation: molecular aspects of dedifferentiation, redifferentiation, transdifferentiation, genetic reprogramming of plant cells in vitro, callus, regeneration, epigenetic modifications
  • 9) Plant single cell „omics“ I: transcriptomics, proteomics, metabolomics, lipidomics, synthetic biology, organellar omics studies
  • 10) Plant single cell „omics“ II: single cell and organellar hormonomics, cells sorting, nuclei sorting, cell organelle isolation, reporter cell lines, "cell atlas"
Study resources and literature
    recommended literature
  • ALBERTS, Bruce; Rebecca HEALD; Alexander JOHNSON; David Owen MORGAN; Martin C. RAFF; Keith ROBERTS and Peter WALTER. Molecular biology of the cell. Edited by John H. Wilson - Tim Hunt. Seventh edition. New York, NY: W. W. Norton & Company, 2022, xxxviii, 1. ISBN 9780393884821. info
  • Plant cells and their organelles. Edited by William V. Dashek - Gurbachan S. Miglani. First published. Chichester: Wiley-Blackwell, 2017, xii, 414. ISBN 9780470976869. info
  • Biochemistry & molecular biology of plants. Edited by Bob B. Buchanan - Wilhelm Gruissem - Russell L. Jones. 2nd edition. Chichester: John Wiley & Sons, 2015, xv, 1264. ISBN 9780470714225. info
Approaches, practices, and methods used in teaching
lectures, discussion with students
Method of verifying learning outcomes and course completion requirements
written test
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
The course is also listed under the following terms Spring 2024, Spring 2025, Spring 2026.
  • Enrolment Statistics (Spring 2027, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2027/C8004