Microscopic Analysis of Urine

Semiquantitative urine analysis is one of the basic biochemical examinations. It consists of urine chemical properties determination by diagnostic strips and morphological examination of urinary elements. Both methods support each other in final determination of the result and both findings should correspond with each other. Urine analysis is required to be performed in one hour and is conducted from single sampling, preferably from the second urination in the morning. Nowadays, the analysis of urine is mostly automated. Manual microscopy is used only for determination of ambiguous or discrepant results.

The urine sediment for manual microscopy is prepared as follows: native urine sample is centrifuged in 2000 rpm and the supernatant is removed and the sediment resuspended to create a tenfold concentrated sample solution.

We use standardized staining (e.g. supravital staining) by Sternheimer for better recognition of elements. Staining reagent consists of 2 dyes (alcian blue and red pyronin B in 1:1 ratio). The staining reagent is added to the concentrated urine sample in 1:10 ratio.

In this database, we have sorted and described 3 variants of pictures.

1. Microscopic findings of stained sediment (10× concentrated urine sample with 400× magnification, Sternheimer staining)
2. Microscopic findings of native sediment (10× concentrated urine sample in 400× magnification)
3. Findings from automatic analyzer iQ 200 (Iris)

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In the following links the database is prepared in a format suitable for print. For better recognition, some elements are magnified.

Authors

	RNDr. Miroslava Beňovská, Ph.D. Department of Laboratory Methods Department of Clinical Biochemistry, University Hospital Brno Personal page in IS MU
	Mgr. Ondřej Wiewiorka Department of Biochemistry – Chemistry Section Department of Clinical Biochemistry, University Hospital Brno Personal page in IS MU
	MUDr. Jana Tůmová Department of Clinical Biochemistry, University Hospital Brno Personal page in IS MU
	Specimen selections
	Team of employees in Department of Clinical Biochemistry, University Hospital Brno