aFABT1_16 Pharmaceutical Biotechnology

Farmaceutická fakulta
jaro 2026

Předmět se v období jaro 2026 nevypisuje.

Rozsah
2/2/0. 28p+28c. 5 kr. Ukončení: zk.
Vyučující
doc. RNDr. Jan Hošek, Ph.D. (přednášející)
doc. RNDr. Jan Hošek, Ph.D. (cvičící)
Ing. Marcela Nejezchlebová (cvičící)
PharmDr. Jakub Treml, Ph.D. (cvičící)
Garance
doc. RNDr. Jan Hošek, Ph.D.
Ústav molekulární farmacie – Ústavy – Farmaceutická fakulta
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Výstupy z učení
Basic knowledge from cytology, genetic engineering, and industrial microbiology which are used in preparation of recombinant pharmaceuticals. Overview of biotechnology production of antibiotics, vitamins, organic acids, amino acids and alkaloids.
Osnova
1) Introduction to pharmaceutical biotechnology, definition, milestones
2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones
3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors
4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification
5) Expression of recombinant proteins in prokaryotic cells and yeasts
6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses
7) The classical biotechnological processes in pharmacology
8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification
9) Examples of classical and recombinant biotechnology products in pharmacy, cytokines, hormones, enzymes, antibodies and their derivates
10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes
11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming
12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry, rules for genetically modified organisms manipulation
13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy
14) New trends in biopharmaceutical products development
Literatura
    doporučená literatura
  • Pharmaceutical biotechnology : fundamentals and applications. Edited by Daan Crommelin - Robert D. Sindelar - Bernd Meibohm. Fifth edition. Cham: Springer, 2019, xxiii, 653. ISBN 9783030007096. info
Výukové metody
lectures, class discussion, practical classes
Metody hodnocení
Written exam
Vyučovací jazyk
Angličtina
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