aFAIA1_13 Instrumental Methods in Medicinal Products Characterization

Farmaceutická fakulta
podzim 2025

Předmět se v období podzim 2025 nevypisuje.

Rozsah
1/1/0. 14p+14c. 3 kr. Ukončení: zk.
Vyučující
doc. Mgr. Jan Muselík, Ph.D. (přednášející)
doc. Mgr. Jan Muselík, Ph.D. (cvičící)
Mgr. Sylvie Pavloková, Ph.D. (cvičící)
Garance
doc. Mgr. Jan Muselík, Ph.D.
Ústav farmaceutické technologie – Ústavy – Farmaceutická fakulta
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
The subject deals with modern instrumental methods useful in the field of pharmaceutical technology, development, and industry. The main aim of this subject is to create a comprehensive overview of methods used for the evaluation, analysis, and quality control of pharmaceutical dosage forms. Main topics: Near and middle infrared spectroscopy, Raman microscopy, dissolution tests and drug content analysis (UV/VIS spectrophotometry, HPLC), light and electron microscopy, laser diffraction analysis, X-ray diffraction analysis, thermal analysis. The theoretical principles of selected methods are discussed, and their practical applications are demonstrated.
Výstupy z učení
After completing the course, the student will be able to: - summarize the main methods used in the analysis of solid dosage forms; - understand the theoretical basis of these methods; - describe the main applications of these methods in pharmaceutical research and industry.
Osnova
1 Middle and near infrared spectroscopy, principles, instrumentation, measurement techniques.
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
2 Applications of infrared spectroscopy, evaluation of spectra
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
3 Dissolution test, methods of determination of active compound content (UV/VIS spectrophotometry).
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
4 Methods of determination of active compound content (HPLC).
Lecturer: Assoc.Prof. Jan Muselík, Ph.D
5 Division of modern microscopic methods; light microscopy: basics of optics, resolution, and magnification of the microscope, lens defects, microscope design, sample preparation, basic methods, measurement principles, use in pharmaceutical technology.
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
6 Electron microscopy: interaction of electrons with the matter, electron source, microscope construction, sample preparation, basic methods (scanning, transmission, and environmental electron microscopy), use in pharmaceutical technology.
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
7 Scanning electron microscopy: standard operating procedure, the influence of parameters on image quality, practical examples.
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
8 Scanning probe microscopy: scanning tunneling microscopy and atomic force microscopy (basic principles, microscope design, measurement modes, use in pharmaceutical technology).
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
9 Determination of particle size and shape: parameters, basic methods (sieve analysis, sedimentation analysis, Coulter particle counters, microscopic techniques, image analysis).
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
10 Methods of particle size determination - laser diffraction
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
11 X-ray analysis, instrumentation and applications in pharmaceutical technology.
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
12 Methods of thermal analysis (differential thermal analysis - DTA, thermogravimetry - TGA, differential scanning calorimetry - DSC).
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
13 Raman microscopy.
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
14 Tutorial giving

SYLABUS OF LABORATORY PRACTISE
1.-2. FT-NIR (evaluation of spectra by chemometric methods).
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
3.-4. Light microscopy in connection with image analysis: work with the device and software, analysis of a model pharmaceutical sample - determination of basic size and shape parameters.
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
5.-6. Optimization of chromatographic conditions, dissolution test of dosage form, chromatographic determination of active compound content.
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
7.-9. Electron microscopy: work with instrument and software, analysis of a model pharmaceutical sample, examples of use in research at DPT.
Lecturer: Mgr. Sylvie Pavloková, Ph.D.
10.-11. Thermal analysis. Calculations.
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
12.-13. Excursion to the laboratory of Raman microscopy.
Lecturer: Assoc.Prof. Jan Muselík, Ph.D.
Literatura
Výukové metody
lectures laboratory practice
Metody hodnocení
Written test
Vyučovací jazyk
Angličtina
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