aFAPB1_16 Pathobiochemistry

Farmaceutická fakulta
jaro 2026

Předmět se v období jaro 2026 nevypisuje.

Rozsah
2/1/0. 28p+14c. 4 kr. Ukončení: zk.
Vyučující
Mgr. Marie Brázdová, Ph.D. (přednášející)
Mgr. Marie Brázdová, Ph.D. (cvičící)
Mgr. Bc. Daniela Nykodýmová, Ph.D. (cvičící)
Garance
Mgr. Marie Brázdová, Ph.D.
Ústav molekulární farmacie – Ústavy – Farmaceutická fakulta
Omezení zápisu do předmětu
Předmět je určen pouze studentům mateřských oborů.
Mateřské obory/plány
Cíle předmětu
Pathobiochemistry follows the course of Biochemistry taught in the winter semester. Biochemistry deals with the metabolic processes in living organisms.
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy.
The aim of the practical exercises are analyzes of some selected diagnostically important compounds, the correct interpretation of the results of analyzes and their use for the purpose of human diagnostic and therapeutic practice.
Výstupy z učení
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy. Advance knowledge of biochemistry forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology and Pharmacogenomics.
Osnova
Syllabus Pathobiochemistry

1. Introduction, the importance of studying pathobiochemistry. The scope and requirements for successful completion of the course exam, recommended literature. Understanding the regulation of metabolism. Biochemical communication.
2. Metabolism disorders, types and causes. Hereditary metabolic diseases. Enzymes, regulation of metabolism. Causes increased activity of cellular enzymes in the plasma. Clinically significant enzymes.
3. Pathobiochemistry of carbohydrates, glucose metabolism and its disorders. Glycemic control disorders. Pathobiochemistry of diabetes mellitus, types of DM. Disorders of glycogen metabolism, glykogenosis.
4. Amino acid metabolism and its disorders. Types of diseases and therapy.
5. Disorders of lipid metabolism. Cholesterol, lipoproteins. Lipidosy, dyslipoproteinaemia.
6. Pathobiochemistry of arteriosclerosis. Ischemic heart failure - cardiac markers.
7. The nucleic acid metabolism disorders of purine and pyrimidine. Hyperuricemia, orotacidurie, therapy.
8. Blood, blood plasma proteins. Blood clotting, coagulopathy. Dysproteinaemias. Porphyrins. Biosynthesis, metabolism disorders. Porphyria, hemoglobinopathies.
9. Xenobiotics and their effects on the body. Detoxification mechanism. Biological oxidation. The effects of free radicals on the organism. Lipoperoxidation antioxidants.
10. Tumor, tumor markers. Basic characteristics of tumor cells. Strategy laboratory tests. Requirements ideal tumor marker. Used tumor markers.
11. Relation between Pathobiochemistry and Clinical Biochemistry. Clinical and biochemical analysis and its specific features. Terminology of Clinical Biochemistry. The analyzed material. Material removal.
12. Analysis of urea and the urinary sediment. Immunochemical methods.
13. Hormones and neurodegenerative disorders. 14. Inflamation.
Sylabus of practical exercises in pathobiochemistry:
1. Exercise: Analysis of tumor suppressor by immunodetection on the membrane.
2. Exercise: Basic biochemical determinations.
3. Exercise: Immunochemical methods.
4. Exercise: Hematological methods. Use of automated hematology analyzers in clinical practice. Coagulation methods.
5. Exercise: Final test
Literatura
    doporučená literatura
  • Murray et al. Harpeŕ´s Illustrated Biochemistry. 29th Edition. Lange, 2012. info
  • Robbins SL, Cotrans RS, Kumar V. The Pathologic Basis of Disease, 3rd Edition. Saunders, 2010. info
Výukové metody
lectures, class discussion, laboratory work, video
Metody hodnocení
The written part (online), which consists of a on-line test. . To successfully complete the course, it is necessary to pass the test at 60%. Prerequisite for passing the exam is to complete all practical exercises, passing a test from practical work at 80%, adequate knowledge of biochemical issues and the correct elaboration of all protocols for exercises. He will then reimburse the teaching at the time and in the manner determined by the respective teacher.
Vyučovací jazyk
Angličtina
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