The course is only offered to the students of the study fields the course is directly associated with.
Fields of study the course is directly associated with
there are 20 fields of study the course is directly associated with, display
The practical course of Cell Biology consists of six thematic blocks of practical tasks, taking up the lecture of Cell Biology. The topics of the practical tasks involve issues such as the transport of substances across the cell membrane, cell locomotion and irritability, mitosis, meiosis, training with light microscope as well as basic laboratory procedures employed in biology laboratories. After this course, students should be able to work with automatic pipettes, to prepare solutions, to know the correct procedures for microscopy, and to prepare simple plant or animal microscopy slides. The course has been designed as a preparation for more specialized courses and is aimed to harmonize unequal levels of students' knowledge from secondary schools.
1. Measuring exact volumes in biology. Manipulation with automatic pipettes and pipetting heads or with glass pipettes: repeated (10 times) pipetting an exact volume of the liquid, weighing and statistical assessment of the precision of laboratory work. Model calculations of solution concentration
2. Manipulation with light microscope, training with immersion objective, measuring biological objects using a stage micrometer and a reticle. Native preparations of parenchyma cells from the Elodea waterweed, permanent slides of blood smears, measuring individual types of blood cells: erythrocytes, leukocytes, thrombocytes from humans and from other class of vertebrates.
3. Transport of substances, osmotic phenomena. Plasmolysis and deplasmolysis in epidermal cells of onion and the Elodea waterweed, dependence of reversibility of this process on the concentration of plasmolytic solution. Observation of pollen grains in hypotonic solution, reaction of erythrocytes in hyper- and hypotonic solutions.
4. Mitosis, modification of mitosis (polyploidy, polyteny). Mitotic figures in onion bulb cells, temporary and permanent slides, staining with aceto-orcein. Mitotic failures – onion bulb after hydrochinon treatment, temporary slide stained with aceto-orcein. Counting the mitotic index in permanent slides of tissue cultures.
Preparation of salivary glands from Chironomidae, comparison of temporary and permanent slide, polyploid nuclei of the silkworm (Bombyx mori).
5. Meiosis. Permanent slides of the ovary and seminiferous tubules, egg cells from the Pelophylax frog, pigs' sperm cells – live spermatozoa and permanent slides.
6. Cell locomotion and irritability. Brownian motion observed in a suspension of ferric oxide particles. Observation of protozoa in a hay infusion – ciliary, amoeboid and flagellar motion. Chemotaxis of protozoa from a solution drop containing NaCl into a drop of pure water through a thin liquid bridge. Oxygenotaxis of protozoa around an air bubble.
PALEČEK, Jiří. Biologie buňky. 1. vyd. Praha: Karolinum, 1996. 120 s. ISBN 8071842664. info
KOPECKÁ, Marie. Lékařská biologie. Praktická cvičení - část druhá : genetika. 1. vyd. Brno: Vydavatelství Masarykovy univerzity, 1995. 70 s. ISBN 80-210-1072-X. info
PALEČEK, Jiří. Obecná zoologie :praktická cvičení. Vyd. 2. Praha: Univerzita Karlova, 1991. 223 s. ISBN 80-7066-493-2. info
KOPECKÁ, Marie. Lékařská biologie : praktická cvičení. 1. vyd. Brno: Masarykova univerzita, 1995. 70 s. ISBN 802101072X. info
laboratory practice, microscopic observation
Sstudents are regularly tested in course of practical exercise, each exercise requires written protocols prepared by attending students; the protocols are assessed by the teacher. Students who did not complete the continuous examination successfully are examined orally at the end of the course – in such case, all topics included in the course are examined.
Language of instruction
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: každý druhý týden 2 hodiny.