Bi7030c Bacterial physiology - practical course

Faculty of Science
Autumn 2025
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
doc. Ivan Kushkevych, Ph.D. (seminar tutor)
Mgr. Matúš Pešta (seminar tutor)
Mgr. Bc. Vít Procházka (seminar tutor)
Guaranteed by
doc. Ivan Kushkevych, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Examination - organic chemistry, biochemistry, microbiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 1/20
fields of study / plans the course is directly associated with
Course objectives
The main objective of the course is to familiarize students with the principles of working with microbial, especially bacterial cultures, and with the help of a set of selected tasks, to equip them with the skills necessary to solve various problems that bacterial physiology can pose. Students will learn to evaluate the obtained experimental data, interpret the results and draw conclusions from them, or, in the event of an experiment failure, identify where an error may have occurred. They will process the obtained results in the form of a protocol.
Learning outcomes
Student will be able to - choose proper conditions for determining the physiological properties of bacteria - create a growth curve of a bacterial culture and calculate basic growth constants from it - compare the enzymatic activity of native cells and enzyme preparations - determine the impact of environmental factors on the viability and vitality of microorganisms
Syllabus
Determination of bacterial proteins. Determination of bacterial dry matter. Growth curve of bacterial cells. Isolation, purification and identification of sulfate-reducing bacteria. Pigments of phototrophic microorganisms. Determination of dehydrogenase activity of native bacterial cells. Preparation of acetone powder with phosphatase activity. Determination of phosphatase activity of acetone powder and native cells. Effect of heavy metals on the growth of bacterial cells.
Literature
  • Schemata pro jednotlivé úlohy poskytne vyučující. Laboratory protocols are provided by teacher.
  • Horáková, D., Němec, M. (2003) : Laboratorní cvičení z fyziologie bakterií (Laboratory protocols from bacterial physiology)
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation at practical course, reports, credit.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2025/Bi7030c