Bi9250cen Special Immunological Methods – Practical Course

Přírodovědecká fakulta
podzim 2024
Rozsah
0/2/0. 2 kr. Ukončení: z.
Vyučováno prezenčně.
Vyučující
doc. RNDr. Milan Číž, Ph.D. (cvičící)
doc. Mgr. Lukáš Kubala, Ph.D. (cvičící)
doc. RNDr. Alena Žákovská, Ph.D. (cvičící)
Garance
doc. Mgr. Lukáš Kubala, Ph.D.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Kontaktní osoba: doc. RNDr. Martin Vácha, Ph.D.
Dodavatelské pracoviště: Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Předpoklady
(! Bi9250c Speciální imunol. met. - cv. ) && ! NOWANY ( Bi9250c Speciální imunol. met. - cv. ) && NOW ( Bi5220en Immunology )
Subject is connected to the basic practice course in immunology that is why students are supposed to have finished the basic practice course in immunology. The course is to extended the theory and praxis of basic immunology subject in its methods.
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
Cíle předmětu
At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;
Výstupy z učení
At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;
Osnova
  • The practice proceeds in the form of individual parts of methods that are solved: 1. putting of antigen on the surface of microtitration plates and their preparation for ELISA test • 2. testing on the presence of antibodies against chosen pathogen in serum • 3. Test for the detection of lymphocyte activity • 4. Imunochromatographic tests - test for the detection of the presence of HIV, RSV antibodies in sera • 5. Test of indirect agglutination. Presence of erytrhrocytes Ag in saliva • 6. Immunocytology - invertebrate immunology, immune cells of insects
Literatura
    doporučená literatura
  • HARLOW, Ed a David LANE. Antibodies : a laboratory manual. New York: Cold Spring Harbor Laboratory. xiii, 726. ISBN 0-87969-314-2. 1988. info
Výukové metody
laboratory exercising; using some of simple and difficult immunological methods.
Metody hodnocení
The students participation at lectures is mandatory. The final classification is in the form of written test at the end of term. The students are recommended to fill in written self-tests offered during the term.
Další komentáře
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
Předmět je zařazen také v obdobích podzim 2021, podzim 2022.