Course Information

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.

Learning outcomes

By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 19. 2. to Sun 26. 5. Tue 8:00–9:50 B11/335

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.

Learning outcomes

By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 8:00–9:50 B11/335

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.

Learning outcomes

By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 8:00–9:50 B11/335

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.

Learning outcomes

By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 1. 3. to Fri 14. 5. Tue 8:00–9:50 online_BCH2

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.

Learning outcomes

By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 8:00–9:50 B11/335

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.

Learning outcomes

By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 18. 2. to Fri 17. 5. Tue 8:00–9:50 B11/335

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Wed 8:00–9:50 B11/235

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Mon 20. 2. to Mon 22. 5. Tue 8:00–9:50 B11/235

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 12:00–13:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 12:00–13:50 B11/235

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Thu 9:00–10:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to understand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Thu 8:00–9:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able tounderstand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 14:00–15:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 21 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able tounderstand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Tue 9:00–10:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able tounderstand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Mon 15:00–16:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able tounderstand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Mon 10:00–11:50 B09/316

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Mon 9:00–10:50 C05/107

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Tue 12:00–13:50 kamenice

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Mon 15:00–16:50 kamenice

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 6) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 7) Mitochondrial respiration and oxidative phosphorylation. 8) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 9) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 10) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 11) Mechanochemical energy conversions. Molecular motors. 12) Bioluminiscence. Bioenergetics of sodium ion. 13) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Timetable

Mon 10:00–11:50 Cpm,02016

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 3 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 3 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Prerequisites (in Czech)

C4182 Biochemistry II || C3580 Biochemistry

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 18 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/2/0. 5 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 18 fields of study the course is directly associated with, display

Syllabus

• An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able tounderstand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able tounderstand and explain the main types of energy transformations in living cells, with emphasis on mechanisms of coupling chemical or photochemical processes with a transport across biological membranes. It should also be able to justify the choice of experimental methods suitable to study a specific bioenergetic problem.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

C8140 Bioenergetics

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 20 fields of study the course is directly associated with, display

Course objectives

An advanced lecture. Energy conservation in living organisms - overview and thermodynamics. Stucture and function of membrane enzymes. Electron transport in respiratory chains and during photosynthesis. Evolution of bioenergetic processes. Bioenergetics and ecology.

Syllabus

• 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.

Literature

• FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992, 255 s. ISBN 0125181248. info
• DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988, 128 pp. skriptum. info
• KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985, 151 pp. info
• PEUSNER, L. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984, 277 s. info

Assessment methods (in Czech)

Jde o jednosemestrovou přednášku s výukou 2 hod týdne. U zkoušky (kolokvia) si student vylosuje trojici otázek, z nichž jedna je zaměřena na kvantitativní aspekty předmětu. Nejprve má vyhrazenu 1 hod na zpracování písemné přípravy, pak následuje pohovor.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C8150 Bioenergetics - seminar
  NOW(C8140)  

• Enrolment Statistics (recent)