PřF:C8140 Bioenergetics - Course Information
C8140 BioenergeticsFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- prof. RNDr. Igor Kučera, DrSc. (lecturer)
- Guaranteed by
- prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Contact Person: prof. RNDr. Igor Kučera, DrSc.
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
- C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry and physical chemistry are recommended.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 21 fields of study the course is directly associated with, display
- Course objectives
- The course aims to provide students with a basic understanding of the principles governing energy conversion in living systems. Emphasis is placed on chemiosmotic mechanisms occurring on specialized energy-transducing membranes in bacteria, animals and plants. The existing connections between bioenergetic processes and biogeochemical cycles of biogenic elements in nature are also mentioned.
- Learning outcomes
- By the end of the course, the student will be able to demonstrate knowledge and understanding of the molecular machinery of respiratory and photosynthetic electron-transport chains, ATP synthase, and other membrane transport systems. The student is expected to have understood the main aspects of the storage and conversion of various forms of energy in cells within a broader ecological context.
- 1) History and the present status of bioenergetics. Energy conversions in living organisms: an overview and thermodynamic description. 2) Macroergic compounds. Mechanisms of substrate-level energy conservation. 3) Biomembranes: lipids, proteins and their mutual interactions. Determination of the structure of membrane-bound proteins. 4) Mechanisms of membrane transport. Transporters, channels, ionophores. Membrane-bound transport ATPases. Rotational catalysis in the proton-translocating ATPase. 5) Enzymes, prosthetic groups and electron carriers involved in important redox reactions. 6) Membrane-bound electron-transport chains. Approaches to the study of the electron-transport chains. Artificial electron donors and acceptors. The coupling of redox reaction with proton gradient formation. 7) Isolation, ultrastructure and metabolic activities of mitochondria. Transport of proteins, inorganic ions and metabolites across the mitochondrial membrane. 8) Mitochondrial respiration and oxidative phosphorylation. 9) Aerobic respiration in chemoorganotrophic and chemolithotrophic bacteria. 10) Anaerobic respiration. Regulation mechanisms in facultative anaerobes. 11) Bacteriorhodopsin-based photosynthesis. Anoxygenic and oxygenic photosynthesis dependent on (bacterio)chlorophyll, cooperation of two photosystems in oxygenic photosynthesis. 12) Metabolic cooperation of mitochondria, chloroplasts and cytoplasm. 13) Mechanochemical energy conversions. Thermogenesis in brown fat tissue. Bioluminiscence. Bioenergetics of sodium ion. 14) Evolution of bioenergetic processes. Bioenergetics and the cycles of biogenic elements in the nature.
- FERGUSON, Stuart J. and David G. NICHOLLS. Bioenergetics 2. 2nd ed. London: Academic Press, 1992. 255 s. ISBN 0125181248. info
- DADÁK, Vladimír and Igor KUČERA. Nové poznatky z bioenergetiky (Recent advances in bioenergetics). Praha: Státní pedagogické nakladatelství, 1988. 128 pp. skriptum. info
- KUČERA, Igor. Řešené úlohy z bioenergetiky (Solved problems from bioenergetics). Praha: Státní pedagogické nakladatelství, 1985. 151 pp. info
- PEUSNER, Leonardo. Základy bioenergetiky. 1. vyd. Bratislava: Alfa, 1984. 277 s. info
- Teaching methods
- Assessment methods
- A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.
- Language of instruction
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Listed among pre-requisites of other courses