PřF:C8202 Proteomics - Course Information
C8202 Proteomics - a basic courseFaculty of Science
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Mgr. Radka Dopitová, Ph.D. (lecturer)
doc. RNDr. Jan Hejátko, Ph.D. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
RNDr. Hana Konečná (lecturer)
Mgr. Gabriela Lochmanová, Ph.D. (lecturer)
Mgr. David Potěšil, Ph.D. (lecturer)
prof. RNDr. Zbyněk Zdráhal, Dr. (lecturer)
- Guaranteed by
- prof. RNDr. Jiří Fajkus, CSc.
National Centre for Biomolecular Research - Faculty of Science
Contact Person: prof. RNDr. Zbyněk Zdráhal, Dr.
Supplier department: National Centre for Biomolecular Research - Faculty of Science
- Mon 18. 2. to Fri 17. 5. Mon 15:00–16:50 B11/335
- Basic knowledge of biochemistry and/or molecular biology is a prerequisite for subscription to the course.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biophysics (programme PřF, N-FY)
- Biochemistry (programme PřF, N-BCH)
- Environmental Chemistry (programme PřF, N-CH)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB)
- Course objectives
- The aim of this subject is an introduction of proteomics as discipline dealing with characterization of proteome and individual proteins; methods for their characterization including higher levels of protein structure. To provide students with outline of methods for preparation of protein samples, basic methods for protein separation and characterization of proteins by mass spektrometry including principles of data processing.
- Learning outcomes
- At the end of the course students should be able to: understand to basics of proteomics explain principles of basic proteomic methods used in subsequent practical course (C8302) propose appropriate approach for various kinds of proteomic experiments understand to interpretation of selected types of proteomic data
- Introduction to proteomics Protein functions/Recombinant proteins Characterization of protein structure Basics of protein sample preparation Methods for separation of proteins and peptides Mass spektrometry (MS) in proteomics Introduction to processing of MS data
- Wilkins et al. Proteome Research: New Frontiers in Functional Genomics. Springer, 1997, ISBN 3-540-62753-7
- Chapman J. R. (Editor). Mass Spectrometry of Proteins and Peptides
- Kinter M., Sherman N.E. Protein Sequencing and Identification Using Tandem Mass Spectrometry Wiley-Interscience, 2000, ISBN 0471322490
- Teaching methods
- The main teaching method are lectures that include particular examples from the own scientific praxis of participating teachers and demonstration of the solution of particular problems via individual tools of recent proteomics.
- Assessment methods
- Lecture will be given in 2-hour units
study materials will be available in IS
- Language of instruction
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2019/C8202