F5370 Biophysical labs by UFKL

Faculty of Science
autumn 2017
Extent and Intensity
0/3. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
Mgr. Karel Kubíček, PhD. (lecturer)
doc. Mgr. Lukáš Trantírek, Ph.D. (lecturer)
RNDr. Silvie Trantírková, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics - Physics Section - Faculty of Science
Contact Person: Mgr. Karel Kubíček, PhD.
Supplier department: Department of Condensed Matter Physics - Physics Section - Faculty of Science
Timetable
Mon 18. 9. to Fri 15. 12. Mon 10:00–12:50 Kontaktujte učitele
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This practical course is focused on protein expression and purification (cytochrome b5 and/or c), and their subsequent characterisation. It is possible to express an alternative protein of student's choice; however, the student has to discuss this with the head of this course.
Learning outcomes
After successful passing of this laboratory course the students should be able to
- list and describe basic operations during protein expression
- list and describe basic optical characterisation techniques of proteins
- apply these methods for a generic protein.
Syllabus
  • 1) cell-growth and OD monitoring
  • 2) centrifugation and sonication
  • 3) vertical and horizontal ELFO
  • 4) purification of the expressed protein using liquid chromatography
  • 5) SERS
  • The student(s) will prepare all buffers and media as well as gels as a part of this course.
  • The protocols and other materials will be provided handed to the student during the first informative course.
Literature
  • Pollock, W.B.R., Rosell, F.I., Twitchett, M.B., Dumont, M.E. & Mauk, A.G. (1998) Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and alkaline conformational transition. Biochemistry 37, 6124-6131.
  • Surface enhanced raman spectroscopy : analytical, biophysical and life science applications. Edited by Sebastian Schlücker. Weinheim: Wiley-VCH Verlag GmbH & CO. KGaA, 2011. 331 s. ISBN 9783527325672. info
Teaching methods
laboratory practice, with continuous checking of the students' preparedness via discussion
Assessment methods
In this practical course, the student is awarded graded credit after a completion of the course with 100% participation with respect to the student's understanding of the process of protein expression and its characterisation.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Teacher's information
http://www.physics.muni.cz/biophys/
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019.
  • Enrolment Statistics (autumn 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2017/F5370