F5370 Biophysical labs by UFKL

Faculty of Science
Autumn 2011
Extent and Intensity
0/3. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
prof. Dr. Jiří Kozelka, PhD. (lecturer)
Mgr. Karel Kubíček, PhD. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics - Physics Section - Faculty of Science
Contact Person: Mgr. Karel Kubíček, PhD.
Timetable
Wed 8:00–10:50 Fpbf,-1017
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This practical course is focused on protein expression and purification (cytochrome b5 and/or c). It is possible to express an alternative protein of student's choice; however, the student has to discuss this with the head of this course.

After successful passing of this laboratory course the students should be able to
- list and describe basic operations during protein expression
- apply these methods for a generic protein.
Syllabus
  • 1) cell-growth and OD monitoring
  • 2) centrifugation and sonication
  • 3) vertical and horizontal ELFO
  • 4) purification of the expressed protein using liquid chromatography
  • The student(s) will prepare all buffers and media as well as gels as a part of this course.
  • The protocols and other materials will be provided handed to the student during the first informative course.
Literature
  • Pollock, W.B.R., Rosell, F.I., Twitchett, M.B., Dumont, M.E. & Mauk, A.G. (1998) Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and alkaline conformational transition. Biochemistry 37, 6124-6131.
Teaching methods
laboratory practice, with continuous checking of the students' preparedness via discussion
Assessment methods
In this practical course, the student is awarded graded credit after a completion of the course with 100% participation with respect to the student's understanding of the process of protein expression.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Teacher's information
http://www.physics.muni.cz/biophys/
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2011/F5370