F5370 Biophysical labs by UFKL

Faculty of Science
Autumn 2008
Extent and Intensity
0/3. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
prof. Dr. Jiří Kozelka, PhD. (lecturer)
Mgr. Karel Kubíček, PhD. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics - Physics Section - Faculty of Science
Contact Person: Mgr. Karel Kubíček, PhD.
Timetable
Tue 13:00–15:50 Fpbf,-1017
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
This practical course is focused on protein expression and purification (cytochrome b5 and/or c). It is possible to express an alternative protein of student's choice, the student has to discuss, however, this with the head of this course.
Syllabus
  • This practical course is focused on protein expression and purification (cytochrome b5 and/or c). It is possible to express an alternative protein of student's choice, the student has to discuss, however, this with the head of this course. Main topics of this course: 1) cell-growth and OD monitoring 2) centrifugation and sonication 3) vertical and horizontal ELFO 4) purification of the expressed protein using liquid chromatography The student(s) will prepare all buffers and media as well as gels as a part of this course. The protocols and other materials will be provided handed to the student during the first informative course.
Literature
  • Pollock, W.B.R., Rosell, F.I., Twitchett, M.B., Dumont, M.E. & Mauk, A.G. (1998) Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and alkaline conformational transition. Biochemistry 37, 6124-6131.
Assessment methods
In this practical course, the student is awarded graded credit after a completion of the course with 100% participation. The student's understanding of the process of protein expression is controlled continuously through discussions during the practical course.
Language of instruction
Czech
Further Comments
The course is taught annually.
Teacher's information
http://www.physics.muni.cz/biophys/
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/F5370