C3002 Biosensors and Nanobiotechnology

Přírodovědecká fakulta
jaro 2027
Rozsah
2/0/0. 2 kr. (plus ukončení). Ukončení: zk.
Vyučováno kontaktně
Vyučující
doc. Mgr. Zdeněk Farka, Ph.D. (přednášející)
Mgr. Jan Přibyl, Ph.D. (přednášející)
Garance
doc. Mgr. Zdeněk Farka, Ph.D.
Ústav biochemie – Chemická sekce – Přírodovědecká fakulta
Dodavatelské pracoviště: Ústav biochemie – Chemická sekce – Přírodovědecká fakulta
Předpoklady
Basic knowledge of biochemistry and biology.
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
Anotace
Introduction of basic principles of biosensors and nanobiotechnology - applications of nanometer sized biological objects in technology, preparation, characterization and bioconjugation of nanoparticles, and studies of biomolecules, cells and other biological objects with the help of scanning probe microscopies.
Výstupy z učení
Student will learn principles of biosensors and nanobiotechnology - applications of nanometer sized biological objects in technology, preparation, characterization and bioconjugation of nanoparticles, and studies of biomolecules, cells and other biological objects with the help of scanning probe microscopies.
Klíčová témata
  1. Definition of the biosensor. Historic overview. Characteristics of ideal biosensors.
  2. Electrochemical biosensors, enzyme electrodes. Potentiometric systems and ISFETs. Amperometric measurement of oxygen, hydrogen peroxide and NADH, biosensors based on oxidases and dehydrogenases. Impedimetric and conductometric biosensors.
  3. Spectrophotometric, fluorimetric, and chemiluminescent sensors, optical fibers. Optical biocatalytic sensors. Bioluminescence.
  4. Affinity biosensors based on indirect detection of labels. Immunosensors.
  5. Hybridization biosensors for detecting nucleic acids and oligonucleotide sequences.
  6. Direct optical affinity sensors. Evanescent wave and surface plasmon resonance utilized for monitoring of bioaffinity interactions in real time.
  7. Immobilization of biomolecules for construction of biosensors. Membrane techniques. Electropolymerization. Activation of sensing surfaces. Covalent immobilization of biomolecules.
  8. Miniaturization and mass production of biosensors. Biosensors in integrated analytical microsystems. Biochips. Commercial biosensors.
  9. Nanostructures. Carbon nanotubes, semiconductor nanoparticles - quantum dots. Metal-based nanostructures - nanowires and bioelectronics. Gold nanoparticles (nanorods, nanocages, nanoshells). Magnetic nanoparticles. Separation, characterization and modification of nanoparticles.
  10. Scanning probe microscopy techniques (STM, AFM, SNOM, SECM). Physical principles, basic and advanced measuring modes. Imaging of bioobjects - from atoms and molecules to cells and tissues. Nanolithography and nanomanipulations.
  11. Nanoparticles for biological labeling and cellular imaging. Nanobiosensors and nanobioanalytical systems. Microfluidics, cell sorting and lab-on-a-chip. Biochips and sensing arrays, nanodeposition of biomolecules.
  12. Medical applications. Cytotoxicity of nanoparticles. Nanostructures in drug discovery, delivery, and controlled release. Nanostructures in cancer research. Nanotechnology for tissue engineering and regenerative therapy.
Studijní zdroje a literatura
  • Nanobiotechnology in diagnosis, drug delivery and treatment. Edited by Mahendra Rai - Mehdi Razzaghi-Abyaneh - Avinash P. Ingle. First published. Hoboken, NJ: Wiley-Blackwell, 2021, xii, 404. ISBN 9781119671770. info
  • Kaushik_Nanobiotechnology_for_sensing_applications_from_lab_to_field_2017
  • Tomar_Nanobiotechnology_Concepts_and_Applications_in_Health_Agriculture_and_Environment_2020
Přístupy, postupy a metody používané ve výuce
Lectures with study materials provided in the Information System.
Způsob ověření výstupů z učení a požadavky na ukončení

Written exam. Scoring above 50% of points is required for the successful completion of the exam.

Vyučovací jazyk
Angličtina
Další komentáře
Předmět je dovoleno ukončit i mimo zkouškové období.
Předmět je vyučován každoročně.
Výuka probíhá každý týden.
Předmět je zařazen také v obdobích jaro 2022, jaro 2023, jaro 2024, jaro 2025, jaro 2026.