C8857 Study of interactions between proteins and DNA

Přírodovědecká fakulta
podzim 2008
Rozsah
1/0/0. 4 kr. (příf plus uk plus > 4). Ukončení: zk.
Vyučující
doc. Mgr. Lumír Krejčí, Ph.D. (přednášející)
Garance
doc. Mgr. Lumír Krejčí, Ph.D.
Národní centrum pro výzkum biomolekul – Přírodovědecká fakulta
Rozvrh
Čt 10:00–10:50 C04/211
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
předmět má 15 mateřských oborů, zobrazit
Cíle předmětu
1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
Osnova
  • 1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
Literatura
  • MEYERS., Robert A. Encyclopedia of Molecular Cell Biology and Molecular Medicine, 2nd Edition. 2004. ISBN 3-527-30545-9. info
  • BRANDEN, Carl a John TOOZE. Introduction to Protein Structure. 1991. ISBN 0-8153-0270-3. info
Metody hodnocení
The course will be ended by test. Each student will have to also deliver home assignment that describes their particular project with the potential implementation of the methods and techniques discussed during the course.
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je vyučován každoročně.
Předmět je zařazen také v obdobích podzim 2007 - akreditace, podzim 2010 - akreditace, podzim 2006, podzim 2007, podzim 2009, podzim 2010, podzim 2011, podzim 2011 - akreditace, podzim 2012, podzim 2013, podzim 2014, podzim 2015, podzim 2016, podzim 2019, podzim 2020, podzim 2021, podzim 2022, podzim 2023.