Bi7420 Modern methods for genome analysis

Faculty of Science
Autumn 2022
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Taught online.
MVDr. Boris Tichý, Ph.D. (lecturer)
Mgr. Vojtěch Bystrý, Ph.D. (lecturer)
doc. MUDr. Mgr. Marek Mráz, Ph.D. (lecturer)
Mgr. Terézia Kurucová (lecturer)
Mgr. Filip Pardy (lecturer)
Guaranteed by
MVDr. Boris Tichý, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Vojtěch Bystrý, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Mon 16:00–17:50 B11/305
Prerequisites (in Czech)
Bi1700 Cell Biology && ( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi4020c Molecular biology - practice || Bi6400 Methods of molecular biology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 40 student(s).
Current registration and enrolment status: enrolled: 14/40, only registered: 0/40, only registered with preference (fields directly associated with the programme): 0/40
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should get familiar with the modern molecular-biology techniques and explain the principles of microarray and next generation sequencing technologies
Learning outcomes
After participating on the course student will be able to - apply CRISPR technology in his research - describe principle of next generation sequencing (NGS) - design NGS experiment - analyze NGS data
  • 1. Microarrays - technological aspects: expression, CGH, SNP arrays, resequencing and protein arrays; 2. Next generation sequencing (NGS) – technological aspects of different NGS platforms 3. Library preparation for NGS 4. Methods for non-coding RNA analysis – miRNA, lncRNA, examples of applications in oncology 5. Data analysis – bioinformatics and statistics 6. Aplications and experiment design
  • Koubková L, Vojtěšek B, Vyzula R. Next generation sequencing -  application in clinical practice. Klin Onkol. 2014;27 Suppl 1:S61-8.
Teaching methods
Assessment methods
written test
Language of instruction
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023.
  • Enrolment Statistics (Autumn 2022, recent)
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