Effect of zinc(II) ions on the expression of pro and
anti-apoptotic factors in high-grade prostate carcinoma cells

a 2012

Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells

BALVAN, Jan; Monika HOLUBOVÁ; Jaromír GUMULEC; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ et al.

Základní údaje

Originální název

Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells

Autoři

BALVAN, Jan; Monika HOLUBOVÁ ; Jaromír GUMULEC; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ ; Vojtěch ADAM; René KIZEK a Michal MASAŘÍK

Vydání

MendelNet 2012, 2012

Další údaje

Jazyk

čeština

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Klíčová slova anglicky

apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy

Změněno: 14. 2. 2013 14:53, doc. MUDr. Jaromír Gumulec, Ph.D.

Anotace

V originále

Several typical characteristics of prostate tissue have been identified including the ability to accumulate zinc(II). However, this feature of prostate cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can significantly contribute to the progression of tumour disease and to the ability of prostate cell lines to metastasize. In this study, we aimed our attention on determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate cell line model. Moreover, we were interested in the mechanisms through which exposure of these cell lines to zinc(II) ions could influence expression of the above-mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation, extremely low expression of p53, high expression of Bcl-2 and no changes in the expression of p53. Our experimental data show different effect of zinc(II) ions on expression of the above-mentioned regulatory genes, which may give us more information on their impact on cancer development and progression with possible using for cancer therapy.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GAP301/10/0356, projekt VaV

Název: Studie participace specifických mechanismů poškození DNA na cytoxicitě cytostatik vůči lidským chemosensitivním a chemorestentním neuroblastomům

Citovat

BALVAN, Jan; Monika HOLUBOVÁ; Jaromír GUMULEC; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ; Vojtěch ADAM; René KIZEK a Michal MASAŘÍK. Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells. In MendelNet 2012. 2012.

@proceedings{1087010,
   author = {Balvan, Jan and Holubová, Monika and Gumulec, Jaromír and Sztalmachová, Markéta and Raudenská, Martina and Adam, Vojtěch and Kizek, René and Masařík, Michal},
   booktitle = {MendelNet 2012},
   keywords = {apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy},
   language = {cze},
   title = {Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells},
   url = {http://mnet.mendelu.cz/mendelnet2012/articles/39_balvan_661.pdf},
   year = {2012}
}

TY  - CONF
ID  - 1087010
AU  - Balvan, Jan - Holubová, Monika - Gumulec, Jaromír - Sztalmachová, Markéta - Raudenská, Martina - Adam, Vojtěch - Kizek, René - Masařík, Michal
PY  - 2012
TI  - Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells
KW  - apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy
UR  - http://mnet.mendelu.cz/mendelnet2012/articles/39_balvan_661.pdf
N2  - Several typical characteristics of prostate tissue have been identified including the ability to accumulate zinc(II). However, this feature of prostate cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can significantly contribute to the progression of tumour disease and to the ability of prostate cell lines to metastasize. In this study, we aimed our attention on determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate cell line model. Moreover, we were interested in the mechanisms through which exposure of these cell lines to zinc(II) ions could influence expression of the above-mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation, extremely low expression of p53, high expression of Bcl-2 and no changes in the expression of p53. Our experimental data show different effect of zinc(II) ions on expression of the above-mentioned regulatory genes, which may give us more information on their impact on cancer development and progression with possible using for cancer therapy.
ER  -

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