Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia
coli isolates from an equine clinic and a horseback riding
centre

J 2011

Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre

DOLEJSKÁ, Monika; Eva DUSKOVA; Jana RYBARIKOVA; Dagmar JANOSZOWSKÁ; Eva ROUBALOVÁ et al.

Základní údaje

Originální název

Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre

Autoři

DOLEJSKÁ, Monika; Eva DUSKOVA; Jana RYBARIKOVA; Dagmar JANOSZOWSKÁ; Eva ROUBALOVÁ; Kateřina DIBĎÁKOVÁ; Gabriela MACECKOVA; Ludmila KOHOUTOVA; Ivan LITERÁK; Jiri SMOLA a Alois ČÍŽEK

Vydání

Journal of Antimicrobial Chemotherapy, OXFORD, Oxford University Press, 2011, 0305-7453

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.068

Označené pro přenos do RIV

DOI

https://doi.org/10.1093/jac/dkq500

UT WoS

000288551300012

Klíčová slova anglicky

antibiotics; horses; ESBLs

Změněno: 21. 2. 2013 15:00, RNDr. Eva Roubalová, Ph.D.

Anotace

V originále

Objectives: The aim of this study was to determine the occurrence of extended-spectrum beta-lactamase (ESBL)producing Escherichia coli at an equine clinic and a horseback riding centre, and to discuss the impact of antimicrobial treatment on resistance selection. Methods: Faeces from horses, environmental smears and flies were sampled at both the clinic and riding centre. Staff at the equine clinic were also examined. The samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) to isolate ESBL-producing E. coli. The presence of bla and qnr genes was tested by PCR, and transferability was determined by conjugation. Replicon typing and restriction analysis of plasmids harbouring ESBL and qnr genes were performed. Results: E. coli with the bla(CTX-M-1) gene were isolated from horses, staff, environmental smears and flies at the two sites. E. coli isolates from the equine clinic harboured an IncHI1 conjugative 235-285 kb plasmid containing bla(CTX-M-1), catA1, strA, sul2 and tet(B) genes. Some of these were positive for qnrS1 and/or qnrB19, and were located on 40 or 45 kb IncN or IncX1 conjugative plasmids. The gene bla(CTX-M-1) in isolates from the riding centre was carried by IncN (30 kb) and IncI1 (85 kb) conjugative plasmids. Horizontal gene transfer seems to be involved in disseminating E. coli with ESBL and qnr genes at the clinic and riding centre. Conclusions: The study illustrates that ESBL-producing E. coli, as well as plasmids carrying ESBL genes of clinical interest, can be easily transferred among horses, humans and flies living in close contact.

Citovat

DOLEJSKÁ, Monika; Eva DUSKOVA; Jana RYBARIKOVA; Dagmar JANOSZOWSKÁ; Eva ROUBALOVÁ; Kateřina DIBĎÁKOVÁ; Gabriela MACECKOVA; Ludmila KOHOUTOVA; Ivan LITERÁK; Jiri SMOLA a Alois ČÍŽEK. Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre. Journal of Antimicrobial Chemotherapy. OXFORD: Oxford University Press, 2011, roč. 66, č. 4, s. 757-764. ISSN 0305-7453. Dostupné z: https://doi.org/10.1093/jac/dkq500.

@article{1088259,
   author = {Dolejská, Monika and Duskova, Eva and Rybarikova, Jana and Janoszowská, Dagmar and Roubalová, Eva and Dibďáková, Kateřina and Maceckova, Gabriela and Kohoutova, Ludmila and Literák, Ivan and Smola, Jiri and Čížek, Alois},
   article_location = {OXFORD},
   article_number = {4},
   doi = {https://doi.org/10.1093/jac/dkq500},
   keywords = {antibiotics; horses; ESBLs},
   language = {eng},
   issn = {0305-7453},
   journal = {Journal of Antimicrobial Chemotherapy},
   title = {Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre},
   volume = {66},
   year = {2011}
}

TY  - JOUR
ID  - 1088259
AU  - Dolejská, Monika - Duskova, Eva - Rybarikova, Jana - Janoszowská, Dagmar - Roubalová, Eva - Dibďáková, Kateřina - Maceckova, Gabriela - Kohoutova, Ludmila - Literák, Ivan - Smola, Jiri - Čížek, Alois
PY  - 2011
TI  - Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre
JF  - Journal of Antimicrobial Chemotherapy
VL  - 66
IS  - 4
SP  - 757-764
EP  - 757-764
PB  - Oxford University Press
SN  - 03057453
KW  - antibiotics
KW  - horses
KW  - ESBLs
N2  - Objectives: The aim of this study was to determine the occurrence of extended-spectrum beta-lactamase (ESBL)producing Escherichia coli at an equine clinic and a horseback riding centre, and to discuss the impact of antimicrobial treatment on resistance selection. Methods: Faeces from horses, environmental smears and flies were sampled at both the clinic and riding centre. Staff at the equine clinic were also examined. The samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) to isolate ESBL-producing E. coli. The presence of bla and qnr genes was tested by PCR, and transferability was determined by conjugation. Replicon typing and restriction analysis of plasmids harbouring ESBL and qnr genes were performed. Results: E. coli with the bla(CTX-M-1) gene were isolated from horses, staff, environmental smears and flies at the two sites. E. coli isolates from the equine clinic harboured an IncHI1 conjugative 235-285 kb plasmid containing bla(CTX-M-1), catA1, strA, sul2 and tet(B) genes. Some of these were positive for qnrS1 and/or qnrB19, and were located on 40 or 45 kb IncN or IncX1 conjugative plasmids. The gene bla(CTX-M-1) in isolates from the riding centre was carried by IncN (30 kb) and IncI1 (85 kb) conjugative plasmids. Horizontal gene transfer seems to be involved in disseminating E. coli with ESBL and qnr genes at the clinic and riding centre. Conclusions: The study illustrates that ESBL-producing E. coli, as well as plasmids carrying ESBL genes of clinical interest, can be easily transferred among horses, humans and flies living in close contact.
ER  -

DOLEJSKÁ, Monika; Eva DUSKOVA; Jana RYBARIKOVA; Dagmar JANOSZOWSKÁ; Eva ROUBALOVÁ; Kateřina DIBĎÁKOVÁ; Gabriela MACECKOVA; Ludmila KOHOUTOVA; Ivan LITERÁK; Jiri SMOLA a Alois ČÍŽEK. Plasmids carrying bla(CTX-M-1) and qnr genes in Escherichia coli isolates from an equine clinic and a horseback riding centre. \textit{Journal of Antimicrobial Chemotherapy}. OXFORD: Oxford University Press, 2011, roč.~66, č.~4, s.~757-764. ISSN~0305-7453. Dostupné z: https://doi.org/10.1093/jac/dkq500.

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