Antibiotic resistance in faecal bacteria (Escherichia coli,
Enterococcus spp.) in feral pigeons

J 2010

Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

RADIMĚŘSKÝ, Tomáš; P FROLKOVA; Dagmar JANOSZOWSKÁ; M DOLEJSKA; P SVEC et al.

Základní údaje

Originální název

Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

Autoři

RADIMĚŘSKÝ, Tomáš; P FROLKOVA; Dagmar JANOSZOWSKÁ; M DOLEJSKA; P SVEC; Eva ROUBALOVÁ; P CIKOVA; A CIZEK a Ivan LITERÁK

Vydání

Journal of Applied Microbiology, Oxford, UK, Blackwell Science Ltd, 2010, 1364-5072

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.365

Označené pro přenos do RIV

DOI

https://doi.org/10.1111/j.1365-2672.2010.04797.x

UT WoS

000282813800020

Klíčová slova anglicky

antimicrobials; Columba livia; Czech Republic; genes; intestinal bacteria; resistance

Změněno: 21. 2. 2013 15:02, RNDr. Eva Roubalová, Ph.D.

Anotace

V originále

Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) in the Czech Republic. Methods and Results: Cloacal swabs of feral pigeons collected in the city of Brno in 2006 were cultivated for antibiotic-resistant E. coli. Resistance genes, class 1 and 2 integrons, and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). The samples were also cultivated for enterococci. Species status of enterococci isolates was determined using repetitive extragenic palindromic-PCR. Resistance genes were detected in resistant enterococci by PCR. E. coli isolates were found in 203 of 247 pigeon samples. Antibiotic resistance was recorded in three (1 center dot 5%, n(E. coli) = 203) isolates. Using agar containing ciprofloxacin, 12 (5%, n(samples) = 247) E. coli strains resistant to ciprofloxacin were isolated. No ESBL-producing E. coli isolates were detected. A total of 143 enterococci were isolated: Ent. faecalis (36 isolates), Ent. faecium (27), Ent. durans (19), Ent. hirae (17), Ent. mundtii (17), Ent. gallinarum (12), Ent. casseliflavus (12) and Ent. columbae (3). Resistance to one to four antibiotics was detected in 45 (31%) isolates. Resistances were determined by tetK, tetL, tetM, tetO, aac(6')aph(2''), ant(4')-Ia, aph(3')-IIIa, ermB, pbp5, vanA and vanC1 genes. Conclusions: Antibiotic-resistant E. coli and Enterococcus spp. occurred in feral pigeons in various prevalences. Significance and Impact of the Study: Feral pigeon should be considered a risk species for spreading in the environment antimicrobial resistant E. coli and enterococci.

Citovat

RADIMĚŘSKÝ, Tomáš; P FROLKOVA; Dagmar JANOSZOWSKÁ; M DOLEJSKA; P SVEC; Eva ROUBALOVÁ; P CIKOVA; A CIZEK a Ivan LITERÁK. Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons. Journal of Applied Microbiology. Oxford, UK: Blackwell Science Ltd, 2010, roč. 109, č. 5, s. 1687-1695. ISSN 1364-5072. Dostupné z: https://doi.org/10.1111/j.1365-2672.2010.04797.x.

@article{1088261,
   author = {Radiměřský, Tomáš and Frolkova, P and Janoszowská, Dagmar and Dolejska, M and Svec, P and Roubalová, Eva and Cikova, P and Cizek, A and Literák, Ivan},
   article_location = {Oxford, UK},
   article_number = {5},
   doi = {https://doi.org/10.1111/j.1365-2672.2010.04797.x},
   keywords = {antimicrobials; Columba livia; Czech Republic; genes; intestinal bacteria; resistance},
   language = {eng},
   issn = {1364-5072},
   journal = {Journal of Applied Microbiology},
   title = {Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons},
   volume = {109},
   year = {2010}
}

TY  - JOUR
ID  - 1088261
AU  - Radiměřský, Tomáš - Frolkova, P - Janoszowská, Dagmar - Dolejska, M - Svec, P - Roubalová, Eva - Cikova, P - Cizek, A - Literák, Ivan
PY  - 2010
TI  - Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons
JF  - Journal of Applied Microbiology
VL  - 109
IS  - 5
SP  - 1687-1695
EP  - 1687-1695
PB  - Blackwell Science Ltd
SN  - 13645072
KW  - antimicrobials
KW  - Columba livia
KW  - Czech Republic
KW  - genes
KW  - intestinal bacteria
KW  - resistance
N2  - Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) in the Czech Republic. Methods and Results: Cloacal swabs of feral pigeons collected in the city of Brno in 2006 were cultivated for antibiotic-resistant E. coli. Resistance genes, class 1 and 2 integrons, and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). The samples were also cultivated for enterococci. Species status of enterococci isolates was determined using repetitive extragenic palindromic-PCR. Resistance genes were detected in resistant enterococci by PCR. E. coli isolates were found in 203 of 247 pigeon samples. Antibiotic resistance was recorded in three (1 center dot 5%, n(E. coli) = 203) isolates. Using agar containing ciprofloxacin, 12 (5%, n(samples) = 247) E. coli strains resistant to ciprofloxacin were isolated. No ESBL-producing E. coli isolates were detected. A total of 143 enterococci were isolated: Ent. faecalis (36 isolates), Ent. faecium (27), Ent. durans (19), Ent. hirae (17), Ent. mundtii (17), Ent. gallinarum (12), Ent. casseliflavus (12) and Ent. columbae (3). Resistance to one to four antibiotics was detected in 45 (31%) isolates. Resistances were determined by tetK, tetL, tetM, tetO, aac(6')aph(2''), ant(4')-Ia, aph(3')-IIIa, ermB, pbp5, vanA and vanC1 genes. Conclusions: Antibiotic-resistant E. coli and Enterococcus spp. occurred in feral pigeons in various prevalences. Significance and Impact of the Study: Feral pigeon should be considered a risk species for spreading in the environment antimicrobial resistant E. coli and enterococci.
ER  -

RADIMĚŘSKÝ, Tomáš; P FROLKOVA; Dagmar JANOSZOWSKÁ; M DOLEJSKA; P SVEC; Eva ROUBALOVÁ; P CIKOVA; A CIZEK a Ivan LITERÁK. Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons. \textit{Journal of Applied Microbiology}. Oxford, UK: Blackwell Science Ltd, 2010, roč.~109, č.~5, s.~1687-1695. ISSN~1364-5072. Dostupné z: https://doi.org/10.1111/j.1365-2672.2010.04797.x.

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