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@proceedings{1107521,
author = {Všianská, Pavla and Říhová, Lucie and Varmužová, Tamara and Suská, Renata and Penka, Miroslav and Hájek, Roman},
booktitle = {12th Euroconference on CLINICAL CELL ANALYSIS},
language = {eng},
title = {Analysis of B-cells and plasma cells in multiple myeloma},
year = {2012}
}
TY - CONF
ID - 1107521
AU - Všianská, Pavla - Říhová, Lucie - Varmužová, Tamara - Suská, Renata - Penka, Miroslav - Hájek, Roman
PY - 2012
TI - Analysis of B-cells and plasma cells in multiple myeloma
N2 - Multiple myeloma (MM) is characterized by the presence of clonal plasma cells (PC) arising from malignant transformed B-cells. It is still not clear which stage of B-cell differentiation is responsible for the development of MM and for eventual relapse after treatment, so nowadays there is an effort to identify the source of myeloma-initiating cells. Aim: Analysis of the phenotypic profile and enumeration of B and PC subpopulations in MM patients. Patients and methods: Total of 38 newly diagnosed MM patients and 18 controls without MM were analysed. Results: There was found significantly lower % of the total CD19+ cells in MM than in control samples for both PB and BM (p <0.05). Numbers of transitional B-cells and naive B-cells were reduced in PB of MM when compared with controls (p <0.05). On the contrary, numbers of preGC, total memory and CD27- switched B-cells were increased in MM (p <0.05). No difference was found when compared % of plasmablasts in both groups. There were found similar results as reduction of naive B-cells and increase of total memory and CD27- switched B-cells in BM of MM (p <0.05). As expected, higher % of PC was found in MM mostly with abnormal phenotype CD19- (p <0.005). Conclusion: Polychromatic flow cytometry is capable to identify minimum of 10 B-cell and PC subpopulations. Reduction of CD19+ cells in MM should be mediated by an increased number of abnormal PCs. The enhancement of isotype-switched memory CD27+ and isotype-switched CD27- B-cell subpopulations in MM patients suggests that some of these could be a potential source of myeloma-initiating cells. Analysis on DNA level will be done.
ER -
VŠIANSKÁ, Pavla, Lucie ŘÍHOVÁ, Tamara VARMUŽOVÁ, Renata SUSKÁ, Miroslav PENKA a Roman HÁJEK. Analysis of B-cells and plasma cells in multiple myeloma. In \textit{12th Euroconference on CLINICAL CELL ANALYSIS}. 2012.
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