a 2013

Haplotype analysis of interleukin-8 polymorphisms in chronic and aggressive periodontitis

BOŘILOVÁ LINHARTOVÁ, Petra; Jan VOKURKA; Hana POSKEROVÁ; Antonín FASSMANN; Lydie IZAKOVIČOVÁ HOLLÁ et al.

Základní údaje

Originální název

Haplotype analysis of interleukin-8 polymorphisms in chronic and aggressive periodontitis

Název anglicky

Haplotype analysis of interleukin-8 polymorphisms in chronic and aggressive periodontitis

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Vydání

Front Immunol, Conference Abstract: 15th International Congress of Immunology (ICI), Milan, Italy, 2013

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

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Ne

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DOI

Klíčová slova anglicky

Interleukin-8; IL8 gene polymorphism; bacterial colonization; chronic periodontitis; aggressive periodontitis

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Mezinárodní význam

Anotace

V originále

Objective: Periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. Interleukin-8 (IL-8) variants are important in the regulation of the inflammatory response. The aim of this study was to analyze four polymorphisms in the IL8 gene and their haplotypes in relation to chronic (CP) and aggressive periodontitis (AgP). Design: A total of 492 unrelated subjects were included in this case-control study. Genomic DNA of 278 patients with CP, 58 patients with AgP, and 156 healthy/non-periodontitis controls were genotyped, using the 5 nuclease TaqMan assay for allelic discrimination, for IL8 (rs4073, rs2227307, rs2227306, rs2227532) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit in a subgroup of subjects (N=114). Results: Allele and genotype frequencies of all investigated IL8 polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (p > 0.05). Nevertheless, the complex analysis revealed differences in IL8 haplotype frequencies. Specifically, the haplotypes A(-251)/T(+396)/T(+781) and T(-251)/G(+396)/C(+781) were significantly less frequent in patients with CP (2.1% versus 5.5%, resp. 4.9%, p < 0.05) and haplotype T(-251)/G(+396)/C(+781) was significantly less frequent in patients with AgP (0.9% versus 4.9%, p < 0.05) than in controls. Conclusions: Although none of the investigated SNPs in the IL8 gene was individually associated with periodontitis, some haplotypes can be protective against CP and AgP in the Czech population. Acknowledgements: This study was supported by the project IGA NT11405-6.

Návaznosti

NT11405, projekt VaV
Název: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu
Investor: Ministerstvo zdravotnictví ČR, Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu