Role of PCNA and TLS polymerases in D-loop extension during
homologous  recombination in humans

J 2013

Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

ŠEBESTA, Marek; Peter BURKOVICS; Szilvia JUHASZ; Sufang ZHANG; Judit SZABO et al.

Základní údaje

Originální název

Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

Autoři

ŠEBESTA, Marek; Peter BURKOVICS; Szilvia JUHASZ; Sufang ZHANG; Judit SZABO; Marietta Y. W. T. LEE; Lajos HARACSKA a Lumír KREJČÍ

Vydání

DNA Repair, AMSTERDAM, ELSEVIER SCIENCE BV, 2013, 1568-7864

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.362

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/13:00066407

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.dnarep.2013.05.001

UT WoS

000323468400001

Klíčová slova anglicky

TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution

Štítky

podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 1. 2014 12:17, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.

Návaznosti

EE2.3.20.0011, projekt VaV

Název: Centrum výzkumu pluripotentních buněk a nestability genomu

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

Citovat

ŠEBESTA, Marek; Peter BURKOVICS; Szilvia JUHASZ; Sufang ZHANG; Judit SZABO; Marietta Y. W. T. LEE; Lajos HARACSKA a Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. DNA Repair. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč. 12, č. 9, s. 691-698. ISSN 1568-7864. Dostupné z: https://doi.org/10.1016/j.dnarep.2013.05.001.

@article{1124595,
   author = {Šebesta, Marek and Burkovics, Peter and Juhasz, Szilvia and Zhang, Sufang and Szabo, Judit and Lee, Marietta Y. W. T. and Haracska, Lajos and Krejčí, Lumír},
   article_location = {AMSTERDAM},
   article_number = {9},
   doi = {https://doi.org/10.1016/j.dnarep.2013.05.001},
   keywords = {TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution},
   language = {eng},
   issn = {1568-7864},
   journal = {DNA Repair},
   title = {Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans},
   volume = {12},
   year = {2013}
}

TY  - JOUR
ID  - 1124595
AU  - Šebesta, Marek - Burkovics, Peter - Juhasz, Szilvia - Zhang, Sufang - Szabo, Judit - Lee, Marietta Y. W. T. - Haracska, Lajos - Krejčí, Lumír
PY  - 2013
TI  - Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
JF  - DNA Repair
VL  - 12
IS  - 9
SP  - 691-698
EP  - 691-698
PB  - ELSEVIER SCIENCE BV
SN  - 15687864
KW  - TLS polymerases
KW  - Homologous recombination
KW  - DNA repair synthesis
KW  - D-loop
KW  - Reconstitution
N2  - Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
ER  -

ŠEBESTA, Marek; Peter BURKOVICS; Szilvia JUHASZ; Sufang ZHANG; Judit SZABO; Marietta Y. W. T. LEE; Lajos HARACSKA a Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. \textit{DNA Repair}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč.~12, č.~9, s.~691-698. ISSN~1568-7864. Dostupné z: https://doi.org/10.1016/j.dnarep.2013.05.001.

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