In vivo effects of microcystins and complex cyanobacterial
biomass on rats (Rattus norvegicus var. alba): Changes in
immunological and haematological parameters

J 2013

In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters

PALÍKOVÁ, Miroslava, Petra ONDRACKOVA, Jan MARES, Ondřej ADAMOVSKÝ, Jiri PIKULA et. al.

Základní údaje

Originální název

In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters

Autoři

PALÍKOVÁ, Miroslava (203 Česká republika), Petra ONDRACKOVA (203 Česká republika), Jan MARES (203 Česká republika), Ondřej ADAMOVSKÝ (203 Česká republika, garant, domácí), Jiri PIKULA (203 Česká republika), Jiří KOHOUTEK (203 Česká republika, domácí), Stanislav NAVRATIL (203 Česká republika), Luděk BLÁHA (203 Česká republika, domácí) a Radovan KOPP (203 Česká republika)

Vydání

Toxicon, OXFORD, Elsevier Science, 2013, 0041-0101

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.581

Kód RIV

RIV/00216224:14310/13:00071070

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.toxicon.2013.06.016

UT WoS

000324722700001

Klíčová slova anglicky

Cyanobacteria; Red blood cells; Immunomodulation; Lymphocyte subpopulations; Wistar albino rats

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 4. 2014 13:57, Ing. Andrea Mikešková

Anotace

V originále

Toxic cyanobacteria represent a serious health and ecological problem in waters worldwide. Some previous toxicological studies investigated effects of isolated microcystins on laboratory rodents including mice and rats. However, much less attention has been paid to more realistic exposure situations such as the effects of MCs accumulated in food. The objectives of the present study were to provide a simple model simulation of the food chain in order to evaluate impacts of microcystins (MCs) on rat immune and haematologicalparameters. Impacts of feeding experimental rats with a diet containing fish meat with and without microcystins and complex toxic biomass have been studied during a 28 day exposure. Red blood cell parameters (RBC counts, haematocrit values, MCH, MCV and MCHC) showed significant differences in experimental groups (p <= 0.05, p <= 0.01) in comparison with the control group. We also detected an immunomodulatory effect in the experimental groups. NK cells and gamma delta+ T lymphocytes were significantly increased in peripheral blood in the group exposed to isolated microcystin in the food. Significant change in the ratio of CD4+ and CD8+ cells (increase of CD4+ and a drop in CD8+) was found in the group with added cyanobacterial biomass with low concentration of MCs. The greatest changes in lymphoid organs were observed in the same groups. There was an increase of spleen subpopulations of gamma delta+ T lymphocytes as well as of IgM+ lymphocytes (B lymphocytes) and CD8+ T lymphocytes. Indeed, the modulation of CD4+ and CD8+ of peripheral lymphocytes was associated with similar changes in thymic lymphocytic subpopulations. In summary, food containing doses of microcystins (or toxic cyanobacterial biomass) induces significant changes in RBC parameters and influence preferably innate part of the immune system represented by NK cells and by gamma-delta T cells, which are known to play role as a bridge between adaptive and innate immune response.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

Citovat

PALÍKOVÁ, Miroslava, Petra ONDRACKOVA, Jan MARES, Ondřej ADAMOVSKÝ, Jiri PIKULA, Jiří KOHOUTEK, Stanislav NAVRATIL, Luděk BLÁHA a Radovan KOPP. In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters. Toxicon. OXFORD: Elsevier Science, 2013, roč. 73, č. 2013, s. 1-8. ISSN 0041-0101. Dostupné z: https://dx.doi.org/10.1016/j.toxicon.2013.06.016.

@article{1159430,
   author = {Palíková, Miroslava and Ondrackova, Petra and Mares, Jan and Adamovský, Ondřej and Pikula, Jiri and Kohoutek, Jiří and Navratil, Stanislav and Bláha, Luděk and Kopp, Radovan},
   article_location = {OXFORD},
   article_number = {2013},
   doi = {http://dx.doi.org/10.1016/j.toxicon.2013.06.016},
   keywords = {Cyanobacteria; Red blood cells; Immunomodulation; Lymphocyte subpopulations; Wistar albino rats},
   language = {eng},
   issn = {0041-0101},
   journal = {Toxicon},
   title = {In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters},
   volume = {73},
   year = {2013}
}

TY  - JOUR
ID  - 1159430
AU  - Palíková, Miroslava - Ondrackova, Petra - Mares, Jan - Adamovský, Ondřej - Pikula, Jiri - Kohoutek, Jiří - Navratil, Stanislav - Bláha, Luděk - Kopp, Radovan
PY  - 2013
TI  - In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters
JF  - Toxicon
VL  - 73
IS  - 2013
SP  - 1-8
EP  - 1-8
PB  - Elsevier Science
SN  - 00410101
KW  - Cyanobacteria
KW  - Red blood cells
KW  - Immunomodulation
KW  - Lymphocyte subpopulations
KW  - Wistar albino rats
N2  - Toxic cyanobacteria represent a serious health and ecological problem in waters worldwide. Some previous toxicological studies investigated effects of isolated microcystins on laboratory rodents including mice and rats. However, much less attention has been paid to more realistic exposure situations such as the effects of MCs accumulated in food. The objectives of the present study were to provide a simple model simulation of the food chain in order to evaluate impacts of microcystins (MCs) on rat immune and haematologicalparameters. Impacts of feeding experimental rats with a diet containing fish meat with and without microcystins and complex toxic biomass have been studied during a 28 day exposure. Red blood cell parameters (RBC counts, haematocrit values, MCH, MCV and MCHC) showed significant differences in experimental groups (p <= 0.05, p <= 0.01) in comparison with the control group. We also detected an immunomodulatory effect in the experimental groups. NK cells and gamma delta+ T lymphocytes were significantly increased in peripheral blood in the group exposed to isolated microcystin in the food. Significant change in the ratio of CD4+ and CD8+ cells (increase of CD4+ and a drop in CD8+) was found in the group with added cyanobacterial biomass with low concentration of MCs. The greatest changes in lymphoid organs were observed in the same groups. There was an increase of spleen subpopulations of gamma delta+ T lymphocytes as well as of IgM+ lymphocytes (B lymphocytes) and CD8+ T lymphocytes. Indeed, the modulation of CD4+ and CD8+ of peripheral lymphocytes was associated with similar changes in thymic lymphocytic subpopulations. In summary, food containing doses of microcystins (or toxic cyanobacterial biomass) induces significant changes in RBC parameters and influence preferably innate part of the immune system represented by NK cells and by gamma-delta T cells, which are known to play role as a bridge between adaptive and innate immune response.
ER  -

PALÍKOVÁ, Miroslava, Petra ONDRACKOVA, Jan MARES, Ondřej ADAMOVSKÝ, Jiri PIKULA, Jiří KOHOUTEK, Stanislav NAVRATIL, Luděk BLÁHA a Radovan KOPP. In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): Changes in immunological and haematological parameters. \textit{Toxicon}. OXFORD: Elsevier Science, 2013, roč.~73, č.~2013, s.~1-8. ISSN~0041-0101. Dostupné z: https://dx.doi.org/10.1016/j.toxicon.2013.06.016.

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