Penetration and encapsulation of larval endoparasitoid,
Exorista larvarum (Diptera: Tachinidae) in the factitious host
Galleria mellonella (Lepidoptera: Pyralidae)

J 2014

Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)

VALIGUROVÁ, Andrea; Veronika MICHALKOVÁ; Peter KONÍK; Mária Luisa DINDO; Milan GELNAR et al.

Základní údaje

Originální název

Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)

Název česky

Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)

Autoři

VALIGUROVÁ, Andrea; Veronika MICHALKOVÁ; Peter KONÍK; Mária Luisa DINDO; Milan GELNAR a Jaromír VAŇHARA

Vydání

Bulletin of Entomological Research, Cambridge, England, CAMBRIDGE UNIV PRESS, 2014, 0007-4853

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.910

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00080579

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

endoparasitoid respiratory funnel haemocyte capsule melanin simulated parasitization

Klíčová slova anglicky

endoparasitoid respiratory funnel haemocyte capsule melanin simulated parasitization

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 2. 2018 14:27, doc. RNDr. Andrea Bardůnek Valigurová, Ph.D.

Anotace

V originále

The tachinid fly Exorista larvarum (L.) (Diptera: Tachinidae) is a polyphagous larval endoparasitoid that deposits its eggs on the host exoskeleton of lepidopteran and tenthredinid larvae. The attachment of larval E. larvarum and the formation of the respiratory funnel were studied during infestation in the last larval instar of the wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). The tachinid larvae burrow through the host integument after hatching, using their robust cephalopharyngeal skeleton, leaving a dark spot at the point of their penetration as a result of host cuticle melanization. Endoparasitoid penetration induces the host cellular defence, resulting in the formation of a haemocyte capsule consisting of multi-cellular sheaths. This enveloping capsule later undergoes melanization, which is mostly obvious towards the posterior part of the endoparasitoid. The endoparasitoid uses the host encapsulation response to build a respiratory funnel from the modified host integument, leading to the host surface. The encapsulated larva remains attached to the respiratory funnel via an anal hook and cuticular spines until fully developed. Additional immunohistochemical analyses were used to study host-parasitoid interactions. Indirect immunofluorescence showed no labelling of potential tachinid antigens and confirmed no effect on the surrounding host tissues. A simulated parasitization with coated polybead microspheres revealed the mortal impact of tachinid antigens to the host. Hosts injected with antigen-coated polybeads died as a consequence of an acute and extensive immunological response to the tachinid antigens and not due to the trauma caused by foreign objects inside their body.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

VALIGUROVÁ, Andrea; Veronika MICHALKOVÁ; Peter KONÍK; Mária Luisa DINDO; Milan GELNAR a Jaromír VAŇHARA. Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae). Bulletin of Entomological Research. Cambridge, England: CAMBRIDGE UNIV PRESS, 2014, roč. 104, č. 2, s. 203-212. ISSN 0007-4853. Dostupné z: https://doi.org/10.1017/S0007485313000655.

@article{1163940,
   author = {Valigurová, Andrea and Michalková, Veronika and Koník, Peter and Dindo, Mária Luisa and Gelnar, Milan and Vaňhara, Jaromír},
   article_location = {Cambridge, England},
   article_number = {2},
   doi = {https://doi.org/10.1017/S0007485313000655},
   keywords = {endoparasitoid respiratory funnel haemocyte capsule melanin simulated parasitization},
   language = {eng},
   issn = {0007-4853},
   journal = {Bulletin of Entomological Research},
   title = {Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)},
   url = {http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9127737},
   volume = {104},
   year = {2014}
}

TY  - JOUR
ID  - 1163940
AU  - Valigurová, Andrea - Michalková, Veronika - Koník, Peter - Dindo, Mária Luisa - Gelnar, Milan - Vaňhara, Jaromír
PY  - 2014
TI  - Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)
JF  - Bulletin of Entomological Research
VL  - 104
IS  - 2
SP  - 203-212
EP  - 203-212
PB  - CAMBRIDGE UNIV PRESS
SN  - 00074853
KW  - endoparasitoid respiratory funnel haemocyte capsule melanin simulated parasitization
UR  - http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9127737
N2  - The tachinid fly Exorista larvarum (L.) (Diptera: Tachinidae) is a polyphagous larval endoparasitoid that deposits its eggs on the host exoskeleton of lepidopteran and tenthredinid larvae. The attachment of larval E. larvarum and the formation of the respiratory funnel were studied during infestation in the last larval instar of the wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). The tachinid larvae burrow through the host integument after hatching, using their robust cephalopharyngeal skeleton, leaving a dark spot at the point of their penetration as a result of host cuticle melanization. Endoparasitoid penetration induces the host cellular defence, resulting in the formation of a haemocyte capsule consisting of multi-cellular sheaths. This enveloping capsule later undergoes melanization, which is mostly obvious towards the posterior part of the endoparasitoid. The endoparasitoid uses the host encapsulation response to build a respiratory funnel from the modified host integument, leading to the host surface. The encapsulated larva remains attached to the respiratory funnel via an anal hook and cuticular spines until fully developed. Additional immunohistochemical analyses were used to study host-parasitoid interactions. Indirect immunofluorescence showed no labelling of potential tachinid antigens and confirmed no effect on the surrounding host tissues. A simulated parasitization with coated polybead microspheres revealed the mortal impact of tachinid antigens to the host. Hosts injected with antigen-coated polybeads died as a consequence of an acute and extensive immunological response to the tachinid antigens and not due to the trauma caused by foreign objects inside their body.
ER  -

VALIGUROVÁ, Andrea; Veronika MICHALKOVÁ; Peter KONÍK; Mária Luisa DINDO; Milan GELNAR a Jaromír VAŇHARA. Penetration and encapsulation of larval endoparasitoid, Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae). \textit{Bulletin of Entomological Research}. Cambridge, England: CAMBRIDGE UNIV PRESS, 2014, roč.~104, č.~2, s.~203-212. ISSN~0007-4853. Dostupné z: https://doi.org/10.1017/S0007485313000655.

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