2013
Characterization of stefis from parasitic helminths
ILGOVÁ, Jana, Eliška ŠRÁMOVÁ, Milan GELNAR, Břetislav KOUDELA, Martin KAŠNÝ et. al.Základní údaje
Originální název
Characterization of stefis from parasitic helminths
Název česky
Charakterizace stefinů parazitických helmintů
Autoři
ILGOVÁ, Jana (703 Slovensko, garant, domácí), Eliška ŠRÁMOVÁ (203 Česká republika, domácí), Milan GELNAR (203 Česká republika, domácí), Břetislav KOUDELA (203 Česká republika) a Martin KAŠNÝ (203 Česká republika, domácí)
Vydání
IX. National Conference of Parasitology with International Participation, 2013
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Bulharsko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/13:00066890
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
stefins; helminths; cystatins
Změněno: 12. 2. 2014 16:21, Mgr. et Mgr. Jana Ilgová, Ph.D.
Anotace
V originále
Stefins together with cystatins and kininogens belong to a large family of cysteine peptidase inhibitors. These molecules participate in regulation of various biological processes and have a crucial role as modulators of host immune response (e.g. antigen processing and presentation, nitric oxide production, etc.). Stefins are primarily located intracellularly, but they can be found in high concentrations also in excretory/secretory products of helminths and thus mediating the parasite-host molecular communication. Our study is focused on molecular, biochemical and immunological characterization of stefins which were identified in four parasitic organisms representing the four main groups of helminths: Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda), Eudiplozoon nipponicum (Monogenea) and Trichinella spiralis (Nematoda). Considering the differences in biology of these parasites (e.g. life cycle, localization, nutrition) we expect possible divergence in stefin functions. We performed phylogenetic analysis of cysteine peptidase inhibitors of various species of organisms derived from databases and our transcriptomic and genomic data. Particular genes of stefins of the four model organisms were selected. Total RNA was isolated, reverse transcription performed and DNA coding stefin genes was amplified using specific primers. Obtained genes will be cloned and prepared in recombinant form for further characterization.
Návaznosti
| GBP505/12/G112, projekt VaV |
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| MUNI/A/0937/2012, interní kód MU |
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