Benzofurazane as a New Redox Label for Electrochemical
Detection of DNA: Towards Multipotential Redox Coding of DNA
Bases

J 2013

Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

BALINTOVÁ, Jana; Medard PLUCNARA; Pavlína VIDLÁKOVÁ; Radek POHL; Luděk HAVRAN et. al.

Základní údaje

Originální název

Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

Autoři

BALINTOVÁ, Jana; Medard PLUCNARA; Pavlína VIDLÁKOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK

Vydání

Chemistry - A European Journal, Weinheim, WILEY-VCH Verlag GmbH & Co. KGaA, 2013, 0947-6539

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.696

Kód RIV

RIV/00216224:14740/13:00067004

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1002/chem.201301868

UT WoS

000324316300025

Klíčová slova česky

DNApolymeráza; elektrochemie; nukleosid trifosfát; sekvenování; voltametrie

Klíčová slova anglicky

DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry

Štítky

DNA, electrochemical detection, rivok, Voltammetry

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 4. 2014 20:37, Olga Křížová

Anotace

V originále

Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.

Návaznosti

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

BALINTOVÁ, Jana; Medard PLUCNARA; Pavlína VIDLÁKOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK. Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases. Chemistry - A European Journal. Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA, 2013, roč. 19, č. 38, s. 12720–12731. ISSN 0947-6539. Dostupné z: https://doi.org/10.1002/chem.201301868.

@article{1169994,
   author = {Balintová, Jana and Plucnara, Medard and Vidláková, Pavlína and Pohl, Radek and Havran, Luděk and Fojta, Miroslav and Hocek, Michal},
   article_location = {Weinheim},
   article_number = {38},
   doi = {https://doi.org/10.1002/chem.201301868},
   keywords = {DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry},
   language = {eng},
   issn = {0947-6539},
   journal = {Chemistry - A European Journal},
   title = {Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04},
   volume = {19},
   year = {2013}
}

TY  - JOUR
ID  - 1169994
AU  - Balintová, Jana - Plucnara, Medard - Vidláková, Pavlína - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
PY  - 2013
TI  - Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
JF  - Chemistry - A European Journal
VL  - 19
IS  - 38
SP  - 12720–12731
EP  - 12720–12731
PB  - WILEY-VCH Verlag GmbH & Co. KGaA
SN  - 09476539
KW  - DNA polymerase
KW  - electrochemistry
KW  - nucleoside triphosphates
KW  - sequencing
KW  - voltammetry
UR  - http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
L2  - http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
N2  - Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
ER  -

BALINTOVÁ, Jana; Medard PLUCNARA; Pavlína VIDLÁKOVÁ; Radek POHL; Luděk HAVRAN; Miroslav FOJTA a Michal HOCEK. Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases. \textit{Chemistry - A European Journal}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co. KGaA, 2013, roč.~19, č.~38, s.~12720–12731. ISSN~0947-6539. Dostupné z: https://doi.org/10.1002/chem.201301868.

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