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@proceedings{1184780,
author = {Ilgová, Jana and Koudela, Břetislav and Gelnar, Milan and Kašný, Martin},
booktitle = {V4 Parasitological meeting},
keywords = {Eudiplozoon nipponicum; Hymenolepis diminuta; Fascioloides magna; Trichinella spiralis; cystatin; inhibitor; PCR RACE; coding sequence},
language = {eng},
isbn = {978-80-968473-7-2},
title = {Exploring the cystatins of helminths},
year = {2014}
}
TY - CONF
ID - 1184780
AU - Ilgová, Jana - Koudela, Břetislav - Gelnar, Milan - Kašný, Martin
PY - 2014
TI - Exploring the cystatins of helminths
SN - 9788096847372
KW - Eudiplozoon nipponicum
KW - Hymenolepis diminuta
KW - Fascioloides magna
KW - Trichinella spiralis
KW - cystatin
KW - inhibitor
KW - PCR RACE
KW - coding sequence
N2 - Parasitic organisms release numerous types of molecules essential for their survival. Cystatins - cysteine peptidase inhibitors (CPI) were determined as important protein molecules which participate in the processes in the host-parasite interface. Their effect can influence the host immune response, particularly the processing and presentation of antigens, production of nitric oxide, etc. The aim of our study is to expand the information about cystatins produced by helminths and to reveal their functions in four selected parasitic model species representing the major groups of helminths. We used bioinformatic tools to analyze the transcriptomic and/or genomic data of Eudiplozoon nipponicum (Monogenea), Hymenolepis diminuta (Cestoda), Fascioloides magna (Trematoda) and Trichinella spiralis (Nematoda) and we searched for the presence of conserved cystatin domains. The selected genes of CPI of diverse families - stefins, cystatins and kininogens were amplified, cloned and sequenced and significant differences in their nucleotide/amino acid primary structure were revealed. The production of recombinant forms of CPI is in process in order to characterize their structures and functions.
ER -
ILGOVÁ, Jana, Břetislav KOUDELA, Milan GELNAR a Martin KAŠNÝ. Exploring the cystatins of helminths. In \textit{V4 Parasitological meeting}. 2014. ISBN~978-80-968473-7-2.
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