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@proceedings{1201893, author = {Vaškovicová, Naděžda and Valigurová, Andrea and Hodová, Iveta and Melicherová, Janka and Krzyžánek, Vladislav}, booktitle = {The 18th International Microscopy Congress}, keywords = {freeze-fracture technique; artefacts; cryoprotectant; fragmentation; morphological structures}, language = {eng}, isbn = {978-80-260-6720-7}, title = {Freezer-fracture technique and artefacts caused by processing conditions.}, year = {2014} }
TY - CONF ID - 1201893 AU - Vaškovicová, Naděžda - Valigurová, Andrea - Hodová, Iveta - Melicherová, Janka - Krzyžánek, Vladislav PY - 2014 TI - Freezer-fracture technique and artefacts caused by processing conditions. SN - 9788026067207 KW - freeze-fracture technique KW - artefacts KW - cryoprotectant KW - fragmentation KW - morphological structures N2 - Freeze-fracture technique is method for visualisation membrane surfaces of cell organelles. This method used cryo-fixation for stabilization a sample. Sample is frozen in nitrogen and cut in the chamber under a vacuum and low temperature. Glycerol is used as a cryoprotectant for observation fine and smooth cellular structures. Although, cryoprotectants serve for the substitution water and elimination ice crystals production, they could affect a form of fracture through biological membranes and could affect interpretation of morphological structures. ER -
VAŠKOVICOVÁ, Naděžda, Andrea VALIGUROVÁ, Iveta HODOVÁ, Janka MELICHEROVÁ a Vladislav KRZYŽÁNEK. Freezer-fracture technique and artefacts caused by processing conditions. In \textit{The 18th International Microscopy Congress}. ISBN~978-80-260-6720-7. 2014.
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