PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN
BIRDS

J 2010

PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN BIRDS

BÁRTOVÁ, Eva; Lenka ZDRAŽILOVÁ DUBSKÁ a Ivan LITERÁK

Základní údaje

Originální název

PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN BIRDS

Autoři

BÁRTOVÁ, Eva; Lenka ZDRAŽILOVÁ DUBSKÁ a Ivan LITERÁK

Vydání

INFORMATION AND COMMUNICATION TECHNOLOGY IN EDUCATION, 2010, OSTRAVA 1, UNIV OSTRAVA, 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

UT WoS

000289741800004

Klíčová slova anglicky

innovation of practical; DNA isolation; PCR; electrophoresis; restriction reaction; molecular biology

Změněno: 15. 10. 2014 14:47, MUDr. RNDr. Michal Řiháček, Ph.D., EuSpLM

Anotace

V originále

During winter semester 2009-2010, new practicals focused on the method of molecular biology were introduced into the syllabus of Biology and Genetics. During practicals, students worked on a complex task of bird sex identification from biologic material. The principle of sex identification in birds is based on restriction analysis of PCR product of the gene CHD (Chromo-Helicase-DNA binding gene). At the beginning of the semester, the students obtained information about an innovation of the practicals and the possibility to examine blood sampled from their parrot or other birds. Otherwise, students were provided with tissues from pheasant male or female. The practicals run parallel in two rooms, both of them equipped with required material and machines. The pipettes, DNA isolation sets, regencies for PCR and restriction reaction and other material were shared by group of 3-4 students, however every student tested one sample. During three lessons, students prepared bird tissues or blood lysates, isolated DNA, prepared PCR and subsequently restriction reaction of PC R product, followed by gel electrophoresis and evaluation of the result in UV light. Students appreciated the innovated practicals, especially the possibility to acquire new experience with molecular biology methods that contributed to their understanding of the principles of described methods together with their application potential. These practicals were financed by the European Social Fund and the State Budget of the Czech Republic by project "Innovation in Animal Protection and Welfare Education", number CZ. 107/2.2.00/07.0165.

Citovat

BÁRTOVÁ, Eva; Lenka ZDRAŽILOVÁ DUBSKÁ a Ivan LITERÁK. PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN BIRDS. INFORMATION AND COMMUNICATION TECHNOLOGY IN EDUCATION, 2010. OSTRAVA 1: UNIV OSTRAVA, 2010, s. 51-53.

@article{1202745,
   author = {Bártová, Eva and Zdražilová Dubská, Lenka and Literák, Ivan},
   article_location = {OSTRAVA 1},
   keywords = {innovation of practical; DNA isolation; PCR; electrophoresis; restriction reaction; molecular biology},
   language = {eng},
   journal = {INFORMATION AND COMMUNICATION TECHNOLOGY IN EDUCATION, 2010},
   title = {PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN BIRDS},
   year = {2010}
}

TY  - JOUR
ID  - 1202745
AU  - Bártová, Eva - Zdražilová Dubská, Lenka - Literák, Ivan
PY  - 2010
TI  - PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN BIRDS
JF  - INFORMATION AND COMMUNICATION TECHNOLOGY IN EDUCATION, 2010
SP  - 51-53
EP  - 51-53
PB  - UNIV OSTRAVA
KW  - innovation of practical
KW  - DNA isolation
KW  - PCR
KW  - electrophoresis
KW  - restriction reaction
KW  - molecular biology
N2  - During winter semester 2009-2010, new practicals focused on the method of molecular biology were introduced into the syllabus of Biology and Genetics. During practicals, students worked on a complex task of bird sex identification from biologic material. The principle of sex identification in birds is based on restriction analysis of PCR product of the gene CHD (Chromo-Helicase-DNA binding gene). At the beginning of the semester, the students obtained information about an innovation of the practicals and the possibility to examine blood sampled from their parrot or other birds. Otherwise, students were provided with tissues from pheasant male or female. The practicals run parallel in two rooms, both of them equipped with required material and machines. The pipettes, DNA isolation sets, regencies for PCR and restriction reaction and other material were shared by group of 3-4 students, however every student tested one sample. During three lessons, students prepared bird tissues or blood lysates, isolated DNA, prepared PCR and subsequently restriction reaction of PC R product, followed by gel electrophoresis and evaluation of the result in UV light. Students appreciated the innovated practicals, especially the possibility to acquire new experience with molecular biology methods that contributed to their understanding of the principles of described methods together with their application potential. These practicals were financed by the European Social Fund and the State Budget of the Czech Republic by project "Innovation in Animal Protection and Welfare Education", number CZ. 107/2.2.00/07.0165.
ER  -

BÁRTOVÁ, Eva; Lenka ZDRAŽILOVÁ DUBSKÁ a Ivan LITERÁK. PRACTICAL LESSONS IN MOLECULAR BIOLOGY: SEX DETERMINATION IN BIRDS. \textit{INFORMATION AND COMMUNICATION TECHNOLOGY IN EDUCATION, 2010}. OSTRAVA 1: UNIV OSTRAVA, 2010, s.~51-53.

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