Cysteine peptidase inhibitors from blood-feeding fish parasite
Eudiploozoon nipponicum (Monogenea).

a 2014

Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea).

ILGOVÁ, Jana, Milan GELNAR a Martin KAŠNÝ

Základní údaje

Originální název

Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea).

Autoři

ILGOVÁ, Jana (703 Slovensko, garant, domácí), Milan GELNAR (203 Česká republika, domácí) a Martin KAŠNÝ (203 Česká republika, domácí)

Vydání

48th Annual Meeting of the ÖGTPM “Emerging Diseases & Migration“ 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/14:00074140

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Eudiplozoon nipponicum; cystatin; recombinant protein expression; immunomodulation

Změněno: 27. 11. 2014 10:54, Mgr. et Mgr. Jana Ilgová, Ph.D.

Anotace

V originále

Blood-feeding monogenean Eudiplozoon nipponicum parasitizing the gills of common carp (Cyprinus carpio) is widely distributed Euroasian ectoparasite from Diplozooidae family. The foregoing research has been oriented toward understanding the morphology and ecology of particular species from this taxonomic group. Currently only a little is known about the mechanisms of host-parasite interactions associated with the functional molecules produced by this parasites. Among these molecules appear also the cystatins - inhibitors of cysteine peptidases. These were previously proven to play a substantial role in manipulation of the host immune response by various parasitic species and thus maximizing the parasite success. By revealing the function of cystatin of Eudiplozoon nipponicum we would like to fill the gap in the present knowledge concerning the molecules from diplozoid parasites. By using the data based on Eudiplozoon nipponicum transcriptome we identified cystatine gene sequence homologous to cystatins of other related platyhelminth species. We designed specific primers, amplified, cloned and sequenced the gene coding cystatin. Currently we are optimizing the recombinant protein expression in E. coli expression system (pET19b vector plasmid, E. coli BL21 strain). Recombinant form of E. nipponicum cystatin will be characterized in detail.

Návaznosti

GAP506/12/1258, projekt VaV

Název: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Investor: Grantová agentura ČR, Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

ILGOVÁ, Jana, Milan GELNAR a Martin KAŠNÝ. Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea). In 48th Annual Meeting of the ÖGTPM “Emerging Diseases & Migration“. 2014.

@proceedings{1210611,
   author = {Ilgová, Jana and Gelnar, Milan and Kašný, Martin},
   booktitle = {48th Annual Meeting of the ÖGTPM “Emerging Diseases & Migration“},
   keywords = {Eudiplozoon nipponicum; cystatin; recombinant protein expression; immunomodulation},
   language = {eng},
   title = {Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea).},
   year = {2014}
}

TY  - CONF
ID  - 1210611
AU  - Ilgová, Jana - Gelnar, Milan - Kašný, Martin
PY  - 2014
TI  - Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea).
KW  - Eudiplozoon nipponicum
KW  - cystatin
KW  - recombinant protein expression
KW  - immunomodulation
N2  - Blood-feeding monogenean Eudiplozoon nipponicum parasitizing the gills of common carp (Cyprinus carpio) is widely distributed Euroasian ectoparasite from Diplozooidae family. The foregoing research has been oriented toward understanding the morphology and ecology of particular species from this taxonomic group. Currently only a little is known about the mechanisms of host-parasite interactions associated with the functional molecules produced by this parasites. Among these molecules appear also the cystatins - inhibitors of cysteine peptidases. These were previously proven to play a substantial role in manipulation of the host immune response by various parasitic species and thus maximizing the parasite success. By revealing the function of cystatin of Eudiplozoon nipponicum we would like to fill the gap in the present knowledge concerning the molecules from diplozoid parasites. By using the data based on Eudiplozoon nipponicum transcriptome we identified cystatine gene sequence homologous to cystatins of other related platyhelminth species. We designed specific primers, amplified, cloned and sequenced the gene coding cystatin. Currently we are optimizing the recombinant protein expression in E. coli expression system (pET19b vector plasmid, E. coli BL21 strain). Recombinant form of E. nipponicum cystatin will be characterized in detail.
ER  -

ILGOVÁ, Jana, Milan GELNAR a Martin KAŠNÝ. Cysteine peptidase inhibitors from blood-feeding fish parasite Eudiploozoon nipponicum (Monogenea). In \textit{48th Annual Meeting of the ÖGTPM “Emerging Diseases \&{} Migration“}. 2014.

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