Phenotypes of Escherichia coli isolated from urine: Differences
between extended-spectrum beta-lactamase producers and
sensitive strains

J 2015

Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum beta-lactamase producers and sensitive strains

ŠIŠKOVÁ, Petra; Lenka ČERNOHORSKÁ; Martina MAHELOVÁ; Kristýna TURKOVÁ; Vladana WOZNICOVÁ et al.

Základní údaje

Originální název

Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum beta-lactamase producers and sensitive strains

Autoři

ŠIŠKOVÁ, Petra; Lenka ČERNOHORSKÁ; Martina MAHELOVÁ; Kristýna TURKOVÁ a Vladana WOZNICOVÁ

Vydání

Journal of Microbiology, Immunology and Infection, Taipei, Elsevier Taiwan, 2015, 1684-1182

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Tchaj-wan

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.955

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00087433

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

biochemistry; Escherichia coli; extended-spectrum beta-lactamase; phenotype

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 8. 2015 14:29, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Background: Escherichia coli is a frequent causative agent of urinary tract infections, and increasing resistance of Escherichia coli to antimicrobials presents a growing challenge. Methods: Here we compare phenotypes of extended-spectrum beta-lactamase (ESBL) producers (n = 220) with a control group of sensitive strains (non-ESBL producers; n = 150). For each strain, we assessed the presence of O25 antigen, hemolysis, biofilm production, sensitivity to antibiotics, and biochemical profile. Results: Compared to the control group, ESBL producers were more frequently O25 positive (6.0% vs. 42.3%) and less frequently hemolytic (34.7% vs. 6.4%). Comparison of biofilm production in brain-heart infusion (BHI) and in BHI with 4% glucose supplementation showed that ESBL-positive strains produced biofilm in BHI with glucose less intensely than the control group (p < 0.05). Most ESBL producers were ciprofloxacin-resistant (91.8%). Biochemical analyses revealed that ESBL producers more frequently utilized inositol, ornithine, sorbitol, melibiose, and saccharose, whereas the control group more frequently used esculin, lysine, arginine, and dulcitol. The control group strains with O25 antigen were more commonly resistant to ciprofloxacin (p < 0.05). Pulsed-field gel electrophoresis results showed higher variability among the control group of sensitive strains. Conclusion: These findings suggest a potential to detect ESBL strains based on virulence factors and biochemical properties, which could be useful in shaping proper empiric antimicrobial therapy, and for initiating such therapy as soon as possible.

Návaznosti

NS9665, projekt VaV

Název: Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Investor: Ministerstvo zdravotnictví ČR, Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Citovat

ŠIŠKOVÁ, Petra; Lenka ČERNOHORSKÁ; Martina MAHELOVÁ; Kristýna TURKOVÁ a Vladana WOZNICOVÁ. Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum beta-lactamase producers and sensitive strains. Journal of Microbiology, Immunology and Infection. Taipei: Elsevier Taiwan, 2015, roč. 48, č. 3, s. 329-334. ISSN 1684-1182. Dostupné z: https://doi.org/10.1016/j.jmii.2014.04.010.

@article{1210670,
   author = {Šišková, Petra and Černohorská, Lenka and Mahelová, Martina and Turková, Kristýna and Woznicová, Vladana},
   article_location = {Taipei},
   article_number = {3},
   doi = {https://doi.org/10.1016/j.jmii.2014.04.010},
   keywords = {biochemistry; Escherichia coli; extended-spectrum beta-lactamase; phenotype},
   language = {eng},
   issn = {1684-1182},
   journal = {Journal of Microbiology, Immunology and Infection},
   title = {Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum beta-lactamase producers and sensitive strains},
   volume = {48},
   year = {2015}
}

TY  - JOUR
ID  - 1210670
AU  - Šišková, Petra - Černohorská, Lenka - Mahelová, Martina - Turková, Kristýna - Woznicová, Vladana
PY  - 2015
TI  - Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum beta-lactamase producers and sensitive strains
JF  - Journal of Microbiology, Immunology and Infection
VL  - 48
IS  - 3
SP  - 329-334
EP  - 329-334
PB  - Elsevier Taiwan
SN  - 16841182
KW  - biochemistry
KW  - Escherichia coli
KW  - extended-spectrum beta-lactamase
KW  - phenotype
N2  - Background: Escherichia coli is a frequent causative agent of urinary tract infections, and increasing resistance of Escherichia coli to antimicrobials presents a growing challenge. Methods: Here we compare phenotypes of extended-spectrum beta-lactamase (ESBL) producers (n = 220) with a control group of sensitive strains (non-ESBL producers; n = 150). For each strain, we assessed the presence of O25 antigen, hemolysis, biofilm production, sensitivity to antibiotics, and biochemical profile. Results: Compared to the control group, ESBL producers were more frequently O25 positive (6.0% vs. 42.3%) and less frequently hemolytic (34.7% vs. 6.4%). Comparison of biofilm production in brain-heart infusion (BHI) and in BHI with 4% glucose supplementation showed that ESBL-positive strains produced biofilm in BHI with glucose less intensely than the control group (p < 0.05). Most ESBL producers were ciprofloxacin-resistant (91.8%). Biochemical analyses revealed that ESBL producers more frequently utilized inositol, ornithine, sorbitol, melibiose, and saccharose, whereas the control group more frequently used esculin, lysine, arginine, and dulcitol. The control group strains with O25 antigen were more commonly resistant to ciprofloxacin (p < 0.05). Pulsed-field gel electrophoresis results showed higher variability among the control group of sensitive strains. Conclusion: These findings suggest a potential to detect ESBL strains based on virulence factors and biochemical properties, which could be useful in shaping proper empiric antimicrobial therapy, and for initiating such therapy as soon as possible.
ER  -

ŠIŠKOVÁ, Petra; Lenka ČERNOHORSKÁ; Martina MAHELOVÁ; Kristýna TURKOVÁ a Vladana WOZNICOVÁ. Phenotypes of Escherichia coli isolated from urine: Differences between extended-spectrum beta-lactamase producers and sensitive strains. \textit{Journal of Microbiology, Immunology and Infection}. Taipei: Elsevier Taiwan, 2015, roč.~48, č.~3, s.~329-334. ISSN~1684-1182. Dostupné z: https://doi.org/10.1016/j.jmii.2014.04.010.

Přehled o publikaci