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@article{1226667,
author = {Bláhová, Lucie and Kohoutek, Jiří and Lebedová, Jana and Bláha, Luděk and Vecera, Z. and Buchtova, M. and Míšek, Ivan and Hilscherová, Klára},
article_location = {HEIDELBERG},
article_number = {24},
doi = {http://dx.doi.org/10.1007/s00216-014-8033-z},
keywords = {Glutathione; LC MS/MS; Tissues; Oxidative stress; Nanoparticle; Cadmium oxide},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical chemistry},
title = {Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles},
volume = {406},
year = {2014}
}
TY - JOUR
ID - 1226667
AU - Bláhová, Lucie - Kohoutek, Jiří - Lebedová, Jana - Bláha, Luděk - Vecera, Z. - Buchtova, M. - Míšek, Ivan - Hilscherová, Klára
PY - 2014
TI - Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles
JF - Analytical and Bioanalytical chemistry
VL - 406
IS - 24
SP - 5867-5876
EP - 5867-5876
PB - SPRINGER HEIDELBERG
SN - 16182642
KW - Glutathione
KW - LC MS/MS
KW - Tissues
KW - Oxidative stress
KW - Nanoparticle
KW - Cadmium oxide
N2 - The paper presents the development of an advanced extraction and fast analytical LC MS/MS method for simultaneous analyses of reduced and oxidized glutathione (GSH and GSSG, respectively) in different animal tissues. The simultaneous determination of GSH and GSSG is crucial because the amount and ratio of both GSH and GSSG may be altered in response to oxidative stress, an important mechanism of toxicity. The method uses the derivatization of free thiol groups in GSH. Its performance was demonstrated for less explored tissues (lung, brain, and liver) in mouse. The combined extraction and analytical method has very low variability and good reproducibility, maximum coefficients of variance for within-run and between-run analyses under 8 %, and low limits of quantification; for GSH and GSSG, these were 0.2 nM (0.06 ng/mL) and 10 nM (6 ng/mL), respectively. The performance of the method was further demonstrated in a model experiment addressing changes in GSH and GSSG concentrations in lung of mice exposed to CdO nanoparticles during acute 72 h and chronic 13-week exposures. Inhalation exposure led to increased GSH concentrations in lung. GSSG levels were in general not affected; nonsignificant suppression occurred only after the longer 13-week period of exposure. The developed method for the sensitive detection of both GSH and GSSG in very low tissue mass enables these parameters to be studied in cases where only a little sample is available, i.e. in small organisms or in small amounts of tissue.
ER -
BLÁHOVÁ, Lucie, Jiří KOHOUTEK, Jana LEBEDOVÁ, Luděk BLÁHA, Z. VECERA, M. BUCHTOVA, Ivan MÍŠEK a Klára HILSCHEROVÁ. Simultaneous determination of reduced and oxidized glutathione in tissues by a novel liquid chromatography-mass spectrometry method: application in an inhalation study of Cd nanoparticles. \textit{Analytical and Bioanalytical chemistry}. HEIDELBERG: SPRINGER HEIDELBERG, 2014, roč.~406, č.~24, s.~5867-5876. ISSN~1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-014-8033-z.
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