Automated Microscopy in Forward Genetic Screening of
Arabidopsis

D 2014

Automated Microscopy in Forward Genetic Screening of Arabidopsis

DOBISOVÁ, Tereza a Jan HEJÁTKO

Základní údaje

Originální název

Automated Microscopy in Forward Genetic Screening of Arabidopsis

Autoři

DOBISOVÁ, Tereza (203 Česká republika, domácí) a Jan HEJÁTKO (203 Česká republika, garant, domácí)

Vydání

New York, Plant Cell Morphogenesis: Methods and Protocols, od s. 53-66, 14 s. 2014

Nakladatel

Humana Press

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14740/14:00080711

Organizační jednotka

Středoevropský technologický institut

ISBN

978-1-62703-642-9

ISSN

DOI

http://dx.doi.org/10.1007/978-1-62703-643-6_4

UT WoS

000326510400005

Klíčová slova anglicky

apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape;

Štítky

kontrola MP, MP, rivok, SCOPUS, WOS

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 4. 2016 09:30, Olga Křížová

Anotace

V originále

Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GAP501/11/1150, projekt VaV

Název: Význam interakcí cytokininů a auxinu v regulaci gravitropismu kořene u Arabidopsis thaliana.

Investor: Grantová agentura ČR, Význam interakcí cytokininů a auxinu v regulaci gravitropismu kořene u Arabidopsis thaliana.

GA13-25280S, projekt VaV

Název: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Grantová agentura ČR, Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Citovat

DOBISOVÁ, Tereza a Jan HEJÁTKO. Automated Microscopy in Forward Genetic Screening of Arabidopsis. In Žárský V. Plant Cell Morphogenesis: Methods and Protocols. New York: Humana Press, 2014, s. 53-66. ISBN 978-1-62703-642-9. Dostupné z: https://dx.doi.org/10.1007/978-1-62703-643-6_4.

@inproceedings{1230832,
   author = {Dobisová, Tereza and Hejátko, Jan},
   address = {New York},
   booktitle = {Plant Cell Morphogenesis: Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-62703-643-6_4},
   editor = {Žárský V.},
   keywords = {apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape;},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-62703-642-9},
   pages = {53-66},
   publisher = {Humana Press},
   title = {Automated Microscopy in Forward Genetic Screening of Arabidopsis},
   url = {http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4},
   year = {2014}
}

TY  - JOUR
ID  - 1230832
AU  - Dobisová, Tereza - Hejátko, Jan
PY  - 2014
TI  - Automated Microscopy in Forward Genetic Screening of Arabidopsis
PB  - Humana Press
CY  - New York
SN  - 9781627036429
KW  - apical meristem
KW  - Arabidopsis thaliana
KW  - branching morphogenesis
KW  - cell communication
KW  - cell function
KW  - cell membrane
KW  - cell shape;
UR  - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
L2  - http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
N2  - Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest.
ER  -

DOBISOVÁ, Tereza a Jan HEJÁTKO. Automated Microscopy in Forward Genetic Screening of Arabidopsis. In Žárský V. \textit{Plant Cell Morphogenesis: Methods and Protocols}. New York: Humana Press, 2014, s.~53-66. ISBN~978-1-62703-642-9. Dostupné z: https://dx.doi.org/10.1007/978-1-62703-643-6\_{}4.

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