VRBSKÝ, Jan, Tamás TEREH, Sergiy KYRYLENKO, Petr DVOŘÁK a Lumír KREJČÍ. MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor. Plos one. San Francisco: Public Library of Science, 2015, roč. 10, č. 6, s. "e0127739", 16 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0127739. |
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@article{1309636, author = {Vrbský, Jan and Tereh, Tamás and Kyrylenko, Sergiy and Dvořák, Petr and Krejčí, Lumír}, article_location = {San Francisco}, article_number = {6}, doi = {http://dx.doi.org/10.1371/journal.pone.0127739}, keywords = {PLURIPOTENT STEM-CELLS; HUMAN SOMATIC-CELLS; SMALL-MOLECULE COMPOUNDS; PRIMORDIAL GERM-CELLS; SELF-RENEWAL; NEURONAL DIFFERENTIATION; PROGENITOR CELLS; MOUSE; INDUCTION; NANOG}, language = {eng}, issn = {1932-6203}, journal = {Plos one}, title = {MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor}, volume = {10}, year = {2015} }
TY - JOUR ID - 1309636 AU - Vrbský, Jan - Tereh, Tamás - Kyrylenko, Sergiy - Dvořák, Petr - Krejčí, Lumír PY - 2015 TI - MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor JF - Plos one VL - 10 IS - 6 SP - "e0127739" EP - "e0127739" PB - Public Library of Science SN - 19326203 KW - PLURIPOTENT STEM-CELLS KW - HUMAN SOMATIC-CELLS KW - SMALL-MOLECULE COMPOUNDS KW - PRIMORDIAL GERM-CELLS KW - SELF-RENEWAL KW - NEURONAL DIFFERENTIATION KW - PROGENITOR CELLS KW - MOUSE KW - INDUCTION KW - NANOG N2 - The possibility of replacing the originally discovered and widely used DNA reprogramming transcription factors is stimulating enormous effort to identify more effective compounds that would not alter the genetic information. Here, we describe the generation of induced pluripotent stem cells (iPSc) from head-derived primary culture of mouse embryonic cells using small chemical inhibitors of the MEK and TGF-beta pathways without delivery of exogenous transcription factors. These iPSc express standard pluripotency markers and retain their potential to differentiate into cells of all germ layers. Our data indicate that head-derived embryonic neural cells might have the reprogramming potential while neither the same primary cells cultivated over five passages in vitro nor a cell population derived from adult brain possesses this capacity. Our results reveal the potential for small molecules to functionally replace routinely used transcription factors and lift the veil on molecular regulation controlling pluripotency. The conditions described here could provide a platform upon which other genome non integrative and safer reprogramming processes could be developed. This work also shows novel potential for developing embryonic neural cells. ER -
VRBSKÝ, Jan, Tamás TEREH, Sergiy KYRYLENKO, Petr DVOŘÁK a Lumír KREJČÍ. MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor. \textit{Plos one}. San Francisco: Public Library of Science, 2015, roč.~10, č.~6, s.~''e0127739'', 16 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0127739.
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