PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE
STRUCTURE LEVEL

a 2015

PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE STRUCTURE LEVEL

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ, Jana ILGOVÁ a Milan GELNAR

Základní údaje

Originální název

PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE STRUCTURE LEVEL

Autoři

KONSTANZOVÁ, Veronika (203 Česká republika, domácí), Božena KOUBKOVÁ (203 Česká republika, domácí), Jana ILGOVÁ (703 Slovensko, domácí) a Milan GELNAR (203 Česká republika, domácí)

Vydání

9th International Symposium on Fish Parasites, 31 August – 4 September 2015, Valencia, Spain, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/15:00081010

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Paradiplozoon homoion Monogenea ultrastruktura trávicí trakt haematinová buňka

Klíčová slova anglicky

Paradiplozoon homoion Monogenea ultrastructure digestive tract haematin cell

Příznaky

Mezinárodní význam

Změněno: 16. 10. 2015 12:36, Mgr. et Mgr. Jana Ilgová, Ph.D.

Anotace

V originále

Diplozoons (Monogenea) are blood-feeding ectoparasites of fish with specific cross body arrangement of two permanently fused adult individuals. Each has two large bucal suckers and a muscular pharynx in the anterior part and an opisthaptor with scleroprotein clamps and hooks in the posterior part of body. Whereas these conspicuous morphological structures have been frequently studied, details of internal morphology and ultrastructure are little known. Specimens of P. homoion were obtained from the gills of common bleak Alburnus alburnus; the fish were caught in the littoral zone of Mušov lowland reservoir (South Moravia, Czech Republic). The fish gills were checked using stereo microscope for the presence of all diplozoid ontogenetic stages. Founded parasites were fixed by 2% osmium tetroxide, dehydrated through ascending acetone series and immediately embedded in a Spurr resin. All sections were obtained by Leica EM UC6i ultramicrotome. Semi-thin sections were stained by toluidine blue and ultra-thin sections were contrasted by uranyl acetate lead citrate. Ultra-thin sections were examined by transmission electron microscope operating at 60 kV (JEOL JEM-1010) equipped with Megaview II software. In this study, the ultrastructural details of the gastrointestinal tract of this worm which represent a unique documentation of specialised structures such as buccal suckers, pharynx with pharyngeal glandular cells, haematin cells and the ultrastructural view of the gut lumen with intact erythrocytes, were documented. Our findings support the hypothesis that the mechanism of P. homoion digestion is predominantly intracellular.

Návaznosti

GAP506/12/1258, projekt VaV

Název: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Investor: Grantová agentura ČR, Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Citovat

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ, Jana ILGOVÁ a Milan GELNAR. PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE STRUCTURE LEVEL. In 9th International Symposium on Fish Parasites, 31 August – 4 September 2015, Valencia, Spain. 2015.

@proceedings{1310419,
   author = {Konstanzová, Veronika and Koubková, Božena and Ilgová, Jana and Gelnar, Milan},
   booktitle = {9th International Symposium on Fish Parasites, 31 August – 4 September 2015, Valencia, Spain},
   keywords = {Paradiplozoon homoion Monogenea ultrastructure digestive tract haematin cell},
   language = {eng},
   title = {PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE STRUCTURE LEVEL},
   year = {2015}
}

TY  - CONF
ID  - 1310419
AU  - Konstanzová, Veronika - Koubková, Božena - Ilgová, Jana - Gelnar, Milan
PY  - 2015
TI  - PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE STRUCTURE LEVEL
KW  - Paradiplozoon homoion Monogenea ultrastructure digestive tract haematin cell
N2  - Diplozoons (Monogenea) are blood-feeding ectoparasites of fish with specific cross body arrangement of two permanently fused adult individuals. Each has two large bucal suckers and a muscular pharynx in the anterior part and an opisthaptor with scleroprotein clamps and hooks in the posterior part of body. Whereas these conspicuous morphological structures have been frequently studied, details of internal morphology and ultrastructure are little known. Specimens of P. homoion were obtained from the gills of common bleak Alburnus alburnus; the fish were caught in the littoral zone of Mušov lowland reservoir (South Moravia, Czech Republic). The fish gills were checked using stereo microscope for the presence of all diplozoid ontogenetic stages. Founded parasites were fixed by 2% osmium tetroxide, dehydrated through ascending acetone series and immediately embedded in a Spurr resin. All sections were obtained by Leica EM UC6i ultramicrotome. Semi-thin sections were stained by toluidine blue and ultra-thin sections were contrasted by uranyl acetate lead citrate. Ultra-thin sections were examined by transmission electron microscope operating at 60 kV (JEOL JEM-1010) equipped with Megaview II software. In this study, the ultrastructural details of the gastrointestinal tract of this worm which represent a unique documentation of specialised structures such as buccal suckers, pharynx with pharyngeal glandular cells, haematin cells and the ultrastructural view of the gut lumen with intact erythrocytes, were documented. Our findings support the hypothesis that the mechanism of P. homoion digestion is predominantly intracellular.
ER  -

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ, Jana ILGOVÁ a Milan GELNAR. PARADIPLOZOON HOMOION (MONOGENEA): DIGESTIVE TRACT ON THE FINE STRUCTURE LEVEL. In \textit{9th International Symposium on Fish Parasites, 31 August – 4 September 2015, Valencia, Spain}. 2015.

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